Arginase-1 精氨酸酶1（兔單克隆抗體）

廣州健侖生物科技有限公司

雄激素中zui主要的形式為睪酮，雄激素在體內(nèi)起著重要的作用。除了與生植相關(guān)外，還具有保持體內(nèi)激素平衡；刺激蛋白質(zhì)合成代謝，促進(jìn)氮沉積和增加肌纖維的數(shù)量和厚度等。通過對青春期前的豬（體重約為15kg）和青春期的豬（體重約為74kg）分組試驗(yàn)，結(jié)果表明公豬的飼料報(bào)酬率比去勢公豬高20%，用睪酮或二氫睪酮處理的去勢公豬，蛋白質(zhì)合成率顯著高于對照去勢公豬，蛋白質(zhì)降解率顯著低于對照去勢公豬（Mulvaney，1984）。James T等（1992）通過用去甲雄三烯醇酮乙酸鹽處理小公牛，結(jié)果發(fā)現(xiàn)經(jīng)處理的小公牛平均日增重高于對照小公牛（P<O.001）。Tarocco C等（1986）對63頭大白公豬從30到130kg活重進(jìn)行表型檢測發(fā)現(xiàn)血液中睪酮濃度與背膘呈顯著相關(guān)。然而雄激素是通過與雄激素受體（Androgen Receptor-AR）結(jié)合而發(fā)揮其功能的，若無雄激素受體，則雄激素對組織無刺激反應(yīng)（refractory）。雄激素受體是雄激素作用的中介物質(zhì)。許多年來歐美一些國家一直在研究雄激素受體基因。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿/細(xì)胞核

克隆號：SP156

同型：IgG

適用組織：石蠟/冰凍

陽性對照：正常肝臟/肝細(xì)胞癌

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時間：60min

Arginase-1 精氨酸酶1（兔單克隆抗體）

生物學(xué)特性
1998年Schellekens和Girbal Neuhause等根據(jù)filaggrin的cDNA序列合成的多肽證實(shí)瓜氨酸殘基是類風(fēng)濕關(guān)節(jié)炎特異的抗中間絲相關(guān)蛋白（filaggrin）抗體識別表位的必需組成。通過對基因文庫中各個序列號的filggrin氨基酸序列進(jìn)行分析，合成了一條以瓜氨酸代替精氨酸的20個左右氨基酸殘基的肽鏈。并通過一定的試驗(yàn)顯示了瓜氨酸是RA患者血清中抗filaggrin相關(guān)抗體識別的主要組成性抗原決定簇成分。在2000年，Schellekens將一條由19個氨基酸殘基組成的瓜氨酸肽鏈中的兩個絲氨酸替換為半胱氨酸，形成與β-轉(zhuǎn)角具有相似結(jié)構(gòu)的二硫鍵，合成環(huán)瓜氨酸肽（cyclic citrullinated peptide,CCP）。采用環(huán)瓜氨酸肽為抗原基質(zhì)用ELISA法檢測RA患者血清中的抗環(huán)瓜氨酸肽抗體，敏感性和特異性均有明顯提高。
研究簡史
抗環(huán)瓜氨酸肽抗體（CCP）ELISA法是1998年Schellkens等建立起來輔助診斷RA的檢測方法，該技術(shù)一出現(xiàn)，立即備受關(guān)注，現(xiàn)已有商品供應(yīng)。按照前絲集蛋白cDNA序列，人工合成可能具有抗原表位的9~19個氨基酸短肽，并經(jīng)胍氨酸化修飾，經(jīng)ELISA篩選出具有抗原性片段。
檢測方法
應(yīng)用ELISA法可定性或定量檢測患者血清中抗CCP抗體。Jansen AL等通過不同類型的多關(guān)節(jié)疾病患者血清中抗CCP抗體的測定，預(yù)測了抗CCP抗體大于50AU/ml即可對早期類風(fēng)濕性關(guān)節(jié)炎有較高的敏感性和特異性。檢測了RA患者抗CCP抗體的水平，結(jié)果也同樣顯示了抗CCP抗體的臨界值為50AU/ml。并且RA患者清血清中抗CCP抗體的平均水平為1100AU/ml*（范圍為：57～3419AU/ml。而對照組抗CCP抗體的平均水平僅為6.8AU/ml（范圍為：1～39AU/ml）。進(jìn)一步證實(shí)了抗CCP抗體對RA患者的診斷價(jià)值。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

Biological characteristics
The 1998 polypeptide synthesized by Schellekens and Girbal Neuhause, based on the cDNA sequence of filaggrin, demonstrated that citrulline residues are essential components of rheumatoid arthritis-specific anti-filaggrin antibody epitope recognition. By analyzing the amino acid sequence of filggrin of each sequence number in the gene library, a peptide chain of about 20 amino acid residues with citrulline instead of arginine was synthesized. And through certain tests showed that citrulline is the major constitutive antigenic determinant of anti-filaggrin-associated antibody in serum of RA patients. In 2000, Schellekens replaced two serines in a citrulline peptide chain consisting of 19 amino acid residues with cysteine ??to form disulfide bonds with a similar structure to β-turn, and synthesized cyclic citrulline Cyclic citrullinated peptide (CCP). Using cyclic citrullinated peptide as antigen matrix, the anti-cyclic citrullinated peptide antibody in serum of patients with RA was detected by ELISA, the sensitivity and specificity were significantly improved.
Brief history of the study
The anti-cyclic citrullinated peptide antibody (CCP) ELISA method was established in 1998 by Schellkens et al to assist in the diagnosis of RA. As soon as the technique became available, there was immediate concern that the current supply of the product was available. According to the pre-silk cDNA sequence, artificial short peptides of 9 to 19 amino acids which may have antigenic epitopes were synthesized and modified by guanidine. Antigenic fragments were selected by ELISA.
Detection method
Anti-CCP antibodies in serum of patients can be detected qualitatively or quantitatively by ELISA. Jansen AL and other anti-CCP antibodies in serum of patients with different types of multi-joint diseases, the anti-CCP antibody predicted greater than 50AU / ml early rheumatoid arthritis can have a higher sensitivity and specificity. The level of anti-CCP antibodies in RA patients was also tested and the results also showed that the cutoff value of anti-CCP antibody was 50 AU / ml. The average level of anti-CCP antibody in serum of RA patients was 1100 AU / ml * (range: 57-3419 AU / ml) while the average level of anti-CCP antibody in control group was only 6.8 AU / ml ml), which further confirmed the diagnostic value of anti-CCP antibody in patients with RA.