CD19 B細(xì)胞、濾泡樹突狀細(xì)胞（克隆EP169）

廣州健侖生物科技有限公司

在正常的淋巴組織中，CD19位于生發(fā)中心細(xì)胞（B細(xì)胞和濾泡樹突狀細(xì)胞）、套區(qū)細(xì)胞和濾泡間散在的細(xì)胞，整體的染色方式類似于CD20和CD22.但是與后兩者不同的是，CD19也表達(dá)于前B細(xì)胞，流式檢測方法還顯示CD19可以在漿細(xì)胞中表達(dá)。在腫瘤組織中，CD19陽性見于絕大多數(shù)的B細(xì)胞腫瘤，漿細(xì)胞淋巴瘤以及T細(xì)胞腫瘤陰性。Masir的研究表明，CD19在14%彌漫性大B細(xì)胞淋巴瘤、30%的T細(xì)胞豐富的B細(xì)胞淋巴瘤和75%的移植后B淋巴增殖性疾病中陰性，在經(jīng)典霍奇金病中的R-S細(xì)胞亦不表達(dá)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號：MRQ-36

同型：IgG

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時間：30-60min

CD19 B細(xì)胞、濾泡樹突狀細(xì)胞（克隆EP169）

（四）設(shè)計實驗方法
實驗方法的確定也應(yīng)該根據(jù)實驗?zāi)康倪M(jìn)行。應(yīng)該包括菌種的選擇、配制何種培養(yǎng)基、需要哪些器材和藥品、怎樣接種、怎樣培養(yǎng)、怎樣觀察結(jié)果等等?？晒﹨⒖嫉膶嶒灧椒ㄈ缦拢?br />1、營養(yǎng)元素對黑曲霉生長的影響
⑴制備培養(yǎng)基：本實驗分組配制培養(yǎng)基，培養(yǎng)基配方見附錄1-8。培養(yǎng)基分裝試管，每管裝量4～5mL，0.1MPa滅菌30min后備用。
⑵孢子懸液制備：取無菌水1支，用接種環(huán)從黑曲霉斜面菌種管中挑取菌體2～3環(huán)，放入水中，充分混勻，備用。
⑶接種：每人取*、缺C、缺N、缺P、缺K和缺Zn培養(yǎng)液各一支，用1 mL無菌吸管按無菌操作法接種黑曲霉孢子懸液0.5 mL。本實驗按每4人取一套培養(yǎng)基不接種作為對照。
⑷觀察：接種后，將培養(yǎng)管置28℃溫度下培養(yǎng)5～7d ,觀察黑曲霉菌絲及孢子生長情況。
2、氧氣對微生物生長的影響
⑴菌懸液制備：用接種環(huán)按無菌操作法取根瘤菌、巴斯德梭菌、大腸桿菌1～2環(huán)分別放入三支無菌水中制成菌懸液。
⑵接種：取已熔化并保溫在50℃左右的培養(yǎng)基6支，用無菌吸管分別及取菌懸液0.2 mL接種到培養(yǎng)管中，每種試驗菌接種2個重復(fù)，接種后立即置振蕩器上混勻，并冷凝。
⑶培養(yǎng)：置培養(yǎng)管于28～30℃下培養(yǎng)3d。
⑷結(jié)果檢查：取出培養(yǎng)管，觀察并記錄每支培養(yǎng)管的上、中、下各層次中細(xì)菌的菌苔大小，菌體集中分布的位置，以了解氧氣對幾種細(xì)菌生長的影響。
3、溫度對微生物生長的影響
⑴接種：取抗原抗體蛋白胨瓊脂斜面培養(yǎng)基8支，用接種環(huán)按無菌操作法分別在斜面上劃線接種大腸桿菌與枯草桿菌，勿劃破培養(yǎng)基。
⑵培養(yǎng)：將已接種的斜面培養(yǎng)基，分別放在4℃、28℃、37℃和45℃四種溫度下培養(yǎng)。
⑶觀察：培養(yǎng)48h、72h后觀察生長狀況，根據(jù)菌苔的大小確定其生長zui適溫度。

CD19

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

(D) design experimental methods
The determination of experimental methods should also be based on experimental purposes. Should include the choice of bacteria, preparation of what kind of medium, what equipment and medicines are needed, how to inoculate, how to c*te, how to observe the results and so on. The experimental methods for reference are as follows:
1, nutrient elements on the growth of Aspergillus niger
⑴ Preparation of medium: The experiment group preparation medium, medium formula see Appendix 1-8. Medium sub-test tube, each tube loading 4 ~ 5mL, 0.1MPa sterilized 30min reserve.
⑵ spore suspension preparation: take sterile water 1, with the inoculation loop from Aspergillus niger bacteria tube picked 2 to 3 rings, into the water, mix well and set aside.
(3) Inoculation: Take a complete, absent C, absent N, absent P, deficient K and Zn-deficient culture medium, and inoculate 0.5 mL of Aspergillus niger spore suspension with sterile 1 mL sterile pipette. In this experiment, a set of culture medium was taken for every 4 persons as a control.
⑷ observation: After inoculation, the culture tube was incubated at 28 ° C for 5 to 7 days to observe the growth of Aspergillus niger and spores.
2, the impact of oxygen on microbial growth
⑴ bacterial suspension preparation: Inoculation loop according to aseptic method to take rhizobium, pasteurized bacteria, Escherichia coli 1 ~ 2 rings were placed in three sterile water to make bacterial suspension.
(2) Inoculation: take 6 mediums that have been melted and kept at about 50 ℃, inoculate them into the culture tube with sterile pipette and take 0.2 mL of the bacterial suspension respectively, and inoculate 2 replicates for each test bacteria and shake immediay after inoculation Mix well and condense.
⑶ culture: set culture tube at 28 ~ 30 ℃ for 3 days.
⑷ results check: remove the culture tube, observe and record each culture tube in the upper, middle and lower levels of bacteria in the lawn size, concentration of bacterial cells in the location to understand the impact of oxygen on the growth of several bacteria.
3, the impact of temperature on microbial growth
⑴ vaccination: take antigen antibody peptone agar slant medium 8, with a ring of vaccination according to aseptic technique respectively slant on the slant inoculated E. coli and Bacillus subtilis, do not scratch the medium.
⑵ culture: the slant culture medium has been inoculated, respectively, at 4 ℃, 28 ℃, 37 ℃ and 45 ℃ cultured at four temperatures.
⑶ observation: 48h, 72h after growth observed, according to the size of the lawn to determine the optimum temperature for growth.