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            化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>行業(yè)專用試劑>生物試劑>CBP60380 MCF-7人乳腺癌細胞

            CBP60380 MCF-7人乳腺癌細胞

            具體成交價以合同協(xié)議為準
            • 公司名稱 南京康佰生物科技有限公司
            • 品牌 cobioer/科佰生物
            • 型號 CBP60380
            • 產(chǎn)地
            • 廠商性質(zhì) 生產(chǎn)廠家
            • 更新時間 2021/9/15 9:16:34
            • 訪問次數(shù) 3568

            聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息,謝謝!


            南京康佰生物科技有限公司是一家專業(yè)提供,進口細胞科研試劑,生命科學研究服務(wù)外包,生物大數(shù)據(jù)庫的綜合型公司。公司在多年的發(fā)展歷程中,整合資源,悉心研發(fā)逐步建立起 "較大腫瘤細胞庫”,"較大STR鑒定比對信息庫”等專業(yè)平臺,同時開發(fā)了分子生物學、蛋白工程、病毒包裝、抗體工程、細胞工程、藥物研發(fā)等一系列平臺,與上海張江藥谷、蘇州納米園、南京高新區(qū)及其他企業(yè)均建立了穩(wěn)定的合作關(guān)系。

            普通細胞株,示蹤細胞株,藥靶細胞株,基因檢測標準品

            供貨周期 現(xiàn)貨 規(guī)格 T-25 Flask
            貨號 CBP60380 應用領(lǐng)域 生物產(chǎn)業(yè)
            主要用途 僅限科研使用

            CBP60380

             

            I. General information

            Synonyms:

            MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL

            Background:

            Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen P2 containment. Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

            Species:

            Homo sapiens, human

            Tissue:

            Mammary gland, breast; derived from metastatic site: pleural effusion

            Disease:

            Breast adenocarcinoma

            Gender:

            Female, 69 years

            Morphology:

            Epithelial-like cells growing as monolayers

            Growth Mode:

            Adherent

            Doubling Time:

            ca. 50 hours range 30-72 hours

            DNA Profile:

            Amelogenin: X
            CSF1PO: 10
            D13S317: 11
            D16S539: 11,12
            D5S818: 11,12/12,12
            D7S820: 8,9
            THO1: 6
            TPOX: 9,12

            vWA: 14,15
            Cobioer’s Cell Line Authentication Service

            Culture Medium:

            MEM + 10% FBS+ 1% Non Essential Amino Acids NEAA + 1mM Sodium Pyruvate NaP+ 10 µg/ml human insulininsulinoptional
            We strongly suggest to purchase the complete medium from Cobioer.

            Cryopreservation medium:

            90% FBS+10%DMSO

            Photo:

             

            Antigen Expression:

            Blood Type O; Rh+

            Receptor Expression:

            Estrogen receptor, expressed

            Genes Expressed:

            Insulin-like growth factor binding proteins IGFBP BP-2; BP-4; BP-5

            Immunology:

            cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

            Molec. Genetics:

            Expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR

            Viruses:

            ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -

            Comments:

            The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.
            Growth of MCF7 cells is inhibited by tumor necrosis factor alpha TNF alpha.

            Secretion of IGFBP's can be modulated by treatment with anti-estrogens.

             



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