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            廈門慧嘉生物科技有限公司
                                                                        
            
										  初級會員 | 第10年									
            
                                    								
            

							
            
						
            
                        

					
            
										
            
						
            
                            							
            
								
            
								
            
									
            18906011628
            
								
            
							
            
                            						
            
					
            
										
				
            
			
            
			
            
				
            
					
				
            
			
            
		
            

		
		 
				
            
			
            
				
			
            
		
            
		
            
			
            
				
            
					
					
            
						
            sRANKL Mouse/重組小鼠可溶性RANK配體
            
						
            
							時間：2016/10/14閱讀：526
							
            
							
				
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            sRANKL Mouse/重組小鼠可溶性RANK配體
             
            SynonymsSoluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.
            IntroductionRANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
            DescriptionsRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa.
            CD254 is purified by proprietary chromatographic techniques.
            SourceEscherichia Coli.
            Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
            FormulationThe protein (1mg/ml) was lyophilized with 10mM Na2PO4, pH 7.5 & 50mM NaCl.
            SolubilityIt is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
            StabilityLyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.
            For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
            Please prevent freeze-thaw cycles.
            Amino acid sequencePAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.
            PurityGreater than 95.0% as determined by SDS-PAGE.
            Biological ActivityMeasured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in “Corning® Osteo Assay Surface 24 Well Plates with Transwell® Permeable Supports- A Useful Tool for Co-Culture Studies” by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.
            UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
            
							
							
							
						
            
					
            
				
            
			
            
		
            
		
		
		
		 



  
    
    				
			

		
    
    
    
    
    
    
    
    
    
    

    

     
     
     
	 




	 
	
	
	

            
	
            
		
            
			
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