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            廈門慧嘉生物科技有限公司
                                                                        
            
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            當(dāng)前位置：> 供求商機(jī)> IL 1 alpha Rat
            
					
            
				
            
			
            
		
            

	
            
		
            
			
            IL 1 alpha Rat
            
		
            
		
            
			
		
            
	
            
	
            
		
            
			
            
				
            
					
              
						
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							IL 1 alpha Rat
						
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            貨物所在地：福建廈門市
            
                                        					
            更新時(shí)間：2024-11-24 21:00:06
            
					
            瀏覽次數(shù)：89
            
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							IL 1 alpha Rat 
            產(chǎn)品編號：CYT-381 
            產(chǎn)品規(guī)格：2μg/10μg/0.1mg

					
            
					
                        
                        Synonyms             Hematopoietin-1, Lymphocyte-activating factor （LAF）, Endogenous Pyrogen （EP）, Leukocyte Endogenous Mediator （LEM）, Mononuclear Cell Factor （MCF）, IL-1 alpha,IL1, IL-1A, IL1F1.         
                    
                        Introduction             Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.         
                    
                        Description             Interleukin-1A Rat Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 155amino acids and having a molecular mass of 17703 Dalton. 
                        The IL-1A is purified by proprietary chromatographic techniques.             		        
                    
                        Source             Escherichia Coli.         
                    
                        Physical Appearance             Sterile Filtered White lyophilized （freeze-dried） powder.         
                    
                        Formulation             The protein was lyophilized from a concentrated （1mg/ml） sterile solution containing 50mM Tris-HCL, pH=8.         
                    
                        Solubility             It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.         
                    
                        Stability             Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
                        Please prevent freeze-thaw cycles.             		        
                    
                        Purity             Greater than 98.0% as determined by（a） Analysis by RP-HPLC.
                        （b） Analysis by SDS-PAGE.             		        
                    
                        Amino acid sequence             The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-His-Ser-Phe.         
                    
                        Biological Activity             The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a Specific Activity of 2 x 108 IU/mg.         
                    
                        Protein content             Protein quantitation was carried out by two independent methods
                        1. UV spectroscopy at 280 nm using the absorbency value of 0.751 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 
                        
                        2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.             		        
                    
                        Usage             ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.         
                
            
                                                                				
            
			
            
		
            
	
            
		



  
    
    				
			

		
    
    
    
    
    
    
    
    
    
    

    

     
     
     
	 




	 
	
	
	

            
	
            
		
            
			
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