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（胺鹽）Spermidine trihydrochloride

說明：抑制神經(jīng)元氧化一氮合成酶(nNOS)活性；能與DNA結(jié)合沉淀DNA，被用于與蛋白質(zhì)相結(jié)合的DNA的純化；刺激T4多聚核苷酸激酶的活性。

別名：三鹽酸精脒； N,-(3-氨基丙基)-1，4-丁二胺 三鹽酸鹽；
鹽酸精脒；Spermidine hydrochloride；N,- (3-Aminopropyl)butane-1,4-diamine hydrochloride

分子式：C 7 H 19 N 3 ·3HCl 分子量：254.63

C A S ＃：334-50-9

外觀：無色或白色結(jié)晶

特性：純度(％)：≥98(TLC)

溶解性：溶于水， 熔點(diǎn)：257-259 ℃

R：36/37/38 S：26-36

儲存條件： −20℃

（胺鹽）Spermidine trihydrochloride結(jié)構(gòu)式：

Spermidine trihydrochloride

Storage Temperature 2-8 °C
Product Description
Molecular Formula: C 7 H 19 N 3
Molecular Weight: 145.2
CAS Number: 124-20-9
Melting Point: 23-25 °C
Density: 0.925 g/ml
Refractive Index: 1.4790 (20 °C)
This product is suitable for molecular biology applications. DNase, RNase, or protease activities were not detected.
Spermidine is biogenic polyamine formed from putrescine, a precursor of spermine. It was first detected in human sperm, but occurs widely in nature.
It is essential in both normal and neoplastic tissue growth.

1Spermidine has a role in cell growth processes 

2 and the formation and interconversion of spermidine in mammalian cells has been reported.

3It has been studied in the regulation of tRNA methyltransferase activity

4and stimulates T4 polynucleotide kinase activity.

5 Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions Spermidine is soluble in water (50 mg/ml), ethanol,
and ether.

6Storage/Stability Spermidine is very hygroscopic and air sensitive.

A solution can be formed for storage by dissolving 1.45 g in 10 ml of water and then sterilizing with a 0.22 μm filter. Store this solution as single-use aliquots at ?20 °C for no more than one month.

7 Spermidine free base should be sterile-filtered and not autoclaved, if a sterile solution is necessary. 

References
1. The Index, 11th ed., Entry# 8698.

2. Janne, J., et al., Polyamines in Rapid Growth and Cancer. Biochim. Biophys. Acta, 473(3-4), 241-293 (1978).

3. Porter, C. W., and Bergeron, R. J., Spermidine Requirement for Cell Proliferation in Eukaryotic
Cells: Structural Specificity and Quantitation.
Science, 219(4588), 1083-1085 (1983).

4. Pegg, A. E., et al., Formation and Interconversion of Putrescine and Spermidine in Mammalian Cells. Adv. Enzyme Regul., 19, 427-451 (1980).

5. Mach, M., et al., Regulation of tRNA Methyltransferase Activities by Spermidine and Putrescine. Inhibition of Polyamine Synthesis and tRNA Methylation by Alpha-methylornithine or 1,3-diaminopropan-2-ol in Dictyosium. Biochem. J.,
202(1), 153-162 (1982).

6. Molecular Cloning: A Laboratory Manual, 3rd ed.,
Sambrook, J. F., et al., Cold Spring Harbor
Laboratory Press (Cold Spring Harbor, NY: 2001),
p. A.435.

7. Molecular Cloning: A Laboratory Manual, 3rd ed.,
Sambrook, J. F., et al., Cold Spring Harbor
Laboratory Press (Cold Spring Harbor, NY: 2001),
p. A1.28.
JSX/ARO/RXR 2/04

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