3D全自動(dòng)外泌體熒光檢測分析系統(tǒng)

法國Abbelight公司開發(fā)的3D全自動(dòng)外泌體熒光檢測分析系統(tǒng)是一款無需純化的、全自動(dòng)的可對單個(gè)外泌體進(jìn)行表征分析的全新設(shè)備。該設(shè)備能夠提供外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達(dá)、單個(gè)外泌體的膜蛋白與生物標(biāo)志物共定位等。操作簡單，結(jié)果可靠。

3D全自動(dòng)外泌體熒光檢測分析系統(tǒng)基于特異性免疫捕獲技術(shù)，允許研究者直接分析特定群體的外泌體。通過單分子定位技術(shù)成像，可以得到單個(gè)外泌體的超分辨成像結(jié)果，尺度可以到20nm。全自動(dòng)外泌體熒光檢測分析系統(tǒng)兼容各種生物樣本，除了純化的外泌體之外，對于血液、尿液、惡性腫瘤、腹水中的外泌體也可直接檢測分析，大大拓展了研究范圍。

應(yīng)用方向及主要特征

超高分辨單外泌體成像

左：2D單個(gè)外泌體成像；右：3D單個(gè)外泌體成像

外泌體粒徑分析：通過團(tuán)簇分析外泌體的整體和亞群的粒徑分布

膜蛋白表征與共定位分析，以CD63，CD81&CD9為例表征其單陽，雙陽和三陽的比例

外泌體內(nèi)容物表征

對比穿膜處理后外泌體中內(nèi)容物aGFP的表達(dá)

觀測細(xì)胞中外泌體的分布情況，研究細(xì)胞中細(xì)胞器或者其他蛋白和外泌體的定位關(guān)系

應(yīng)用案例

法國Abbelight公司開發(fā)的3D全自動(dòng)外泌體熒光檢測分析系統(tǒng)是一款無需純化的、全自動(dòng)的可對單個(gè)外泌體進(jìn)行表征分析的全新設(shè)備。該設(shè)備能夠提供單個(gè)外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達(dá)、單個(gè)外泌體的膜蛋白與生物標(biāo)志物共定位等。

應(yīng)用領(lǐng)域：腫瘤診斷，藥系統(tǒng)開發(fā)，眼科疾病診斷，疫苗研發(fā)，脊髓受傷機(jī)制研究，血漿/血清外泌體分析，外泌體工程化，呼吸疾病診斷

測試數(shù)據(jù)

外泌體2D成像

外泌體3D成像

外泌體形態(tài)分析

發(fā)表文章

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