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            OVCAR-3 人卵巢腺癌細(xì)胞的介紹
            
						
            
							時(shí)間：2014-8-22閱讀：4405
						
            
						
            
							 
            OVCAR-3 人卵巢腺癌細(xì)胞的介紹
            形態(tài)特性：上皮細(xì)胞樣
            生長(zhǎng)特性： 貼壁生長(zhǎng)
            特征特性：OVCAR-3細(xì)胞源自一名60歲白人女性卵巢腫瘤組織，由T.C.Hamiltonyu建系于1982年。該細(xì)胞帶有雌、雄激素受體。對(duì)阿霉素，順氯氨鉑，(左旋)苯丙氨酸氮芥有一定抗藥性，適用于卵巢癌的抗藥性研究。OVCAR-3染色體數(shù)量在亞三倍體范圍內(nèi)。
            傳代方法： 1:3傳代,2-3天傳一代
            支原體檢測(cè)：陰性
            此細(xì)胞為我公司走ATCC保藏中心引進(jìn)，以下是ATCC介紹
            NIH:OVCAR-3 [OVCAR3] (ATCC® HTB-161™)
             
            OrganismHomo sapiens, human
            Tissueovary
            Cell Typeepithelial
            Product Formatfrozen
            Morphologyepithelial
            Culture Propertiesadherent
            Biosafety Level1
            Diseaseadenocarcinoma
            Age60 years
            Genderfemale
            EthnicityCaucasian
            Applications
            This cell line is a suitable transfection host.

            NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
            Storage Conditions
            liquid nitrogen vapor temperature
            Derivation
            The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.
            Clinical Data
            Caucasian
            female
            60 years
            Receptor ExpressionAndrogen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
            TumorigenicYes
            Effects
            Yes, Forms colonies in soft agar
            Yes, in nude mice inoculated subcutaneously with 10(7) cells
            (Tumors developed within 21 days at 100% frequency (5/5).)
            Comments
            Forms colonies in soft agar and has an abnormal karyotype.
            Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.
            Both cultured cells and xenografts exhibit androgen and estrogen receptors.
            Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.
            KaryotypeThe cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.
            
							
						
            
					
            
				
            
			
            
		
            
		
	        




 
    
    		
    

		
    
    
    
    
    
    
    
    
    
    

    

     
     
     
	 






	

            
	
            
		
            
			
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