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            TOV-21G 人卵巢癌細(xì)胞
            
						
            
							時(shí)間：2016-8-1閱讀：2589
						
            
						
            
							 
            Designations:TOV-21G
            Depositors: University of Montreal
            Biosafety Level:1
            Shipped:frozen
            Medium & Serum:See Propagation
            Growth Properties:adherent
            Organism:Homo sapiens deposited as human
            Morphology:epithelial
             
            Source:Organ: ovary 
            Tumor Stage: grade 3, stage III 
            Disease: primary malignant adenocarcinoma; clear cell carcinoma
            Cellular Products:keratin
            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
            Applications:Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
            Tumorigenic:Yes
            Oncogene:p53 + (wild type)
            DNA Profile (STR):Amelogenin: X 
            CSF1PO: 13,15 
            D13S317: 11,12 
            D16S539: 10,12 
            D5S818: 12,13 
            D7S820: 12 
            THO1: 7,9.3 
            TPOX: 8,11 
            vWA: 17
            Cytogenetic Analysis:47, XX, +10 [49408 ]
            Age:62 years
            Gender:female
            Comments:This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
            Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
            Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
            • fetal bovine serum to a final concentration of 15%

            • Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
              Temperature: 37.0°C
            Subculturing:Protocol:
            1. Remove and discard culture medium.
            2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
            3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
            4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            5. Add appropriate aliquots of the cell suspension to new culture vessels.
            6. Incubate cultures at 37?C.

            Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended 
            Medium Renewal: Every 3 to 4 days
            Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
            Storage temperature: liquid nitrogen vapor phase
            Related Products:recommended serum:ATCC 30-2020
            References:
            42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
            49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
            CoC1人卵巢癌細(xì)胞系Lec1倉(cāng)鼠卵巢細(xì)胞
            A2780人卵巢癌細(xì)胞OVAC卵巢癌細(xì)胞
            Anglne人卵巢癌細(xì)胞系NIH:OVCAR-3人卵巢癌細(xì)胞
            Caov－3人乳突狀卵巢腺癌細(xì)胞PA-1人卵巢畸胎瘤細(xì)胞
            CHO中國(guó)倉(cāng)鼠卵巢細(xì)胞Pr-HNV8菜青蟲(chóng)卵巢細(xì)胞
            CHO/dhFr-倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷RP70-a-CHO-0.8MTX中國(guó)倉(cāng)鼠卵巢細(xì)胞
            CHO-K1中國(guó)倉(cāng)鼠卵巢細(xì)胞SF9晚秋粒蟲(chóng)卵巢細(xì)胞
            CHO-pt轉(zhuǎn)血小板基因倉(cāng)鼠卵巢細(xì)胞SKOV3人卵巢癌細(xì)胞
            CHO-S中國(guó)倉(cāng)鼠卵巢懸浮細(xì)胞SKOV-3/DDP卵巢癌順鉑耐藥株
            CoC1人卵巢癌細(xì)胞系SW1910人卵巢癌細(xì)胞
            CoC2 卵巢癌 SW626人卵巢腺癌細(xì)胞
            CTLA4 Ig-24中國(guó)倉(cāng)鼠卵巢細(xì)胞TOV-21G人卵巢癌
            ES-2人卵巢透明細(xì)胞癌ZA1轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞
            HO-8910人卵巢癌細(xì)胞ZA6轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞
            HO-8910PM人高轉(zhuǎn)移卵巢癌細(xì)胞 Cho-K1倉(cāng)鼠卵巢細(xì)胞亞株
            HOC1卵巢癌細(xì)胞SK-OV-3人卵巢癌細(xì)胞
            Lec1倉(cāng)鼠卵巢細(xì)胞Sf9昆蟲(chóng)卵巢細(xì)胞
            CHO-K1倉(cāng)鼠卵巢細(xì)胞亞株ES-2人卵巢透明細(xì)胞癌
            CHO/dhFr-倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷SK-OV-3人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞TOV-21G 人卵巢
            
							
						
            
					
            
				
            
			
            
		
            
		
	        




 
    
    		 
     
    
    

    

     
      
     
	 

 


	

            
	
            
		
            
			
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