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            當前位置：上海復祥生物科技有限公司>>細胞庫>>人正常細胞>>CRL-11005FL62891細胞， 人肝細胞
            
					
            
				
            
			
            
		
            
		
            
			
            
				
            
					
            FL62891細胞， 人肝細胞 
            
					
            
																								
						
						  
					
            
					
					
            
                        						    
            
							
															
            參   考   價：
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            具體成交價以合同協(xié)議為準
            
						
            
                        						
            
							
            產(chǎn)品型號：CRL-11005
            
							
            品       牌：其他品牌
            
                            
            廠商性質(zhì)：生產(chǎn)商
            
                            							
            所  在  地：上海市
            
						
            
						
						
						
            
                        							
            更新時間：2025-03-26 09:10:52瀏覽次數(shù)：1891
            
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                                    供貨周期
                  一周
                                                                            規(guī)格
                  凍干粉
                                  
            
                                                                      
            
                                    貨號
                  ATCC 700323
                                                                            應用領域
                  醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
                                  
            
                                                                      
            
                                    主要用途
                  科學研究
                                                
            
        
            
						
            
FL62891細胞， 人肝細胞
            原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！
            
						
						
            
							
            FL62891細胞， 人肝細胞
            ATCC® Number:CRL-11005™    Price:$353.00
            Designations:FL 62891

            Depositors:ImClone Systems Inc.

            Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

            Shipped:frozen

            Medium & Serum:See Propagation

            Growth Properties:adherent

            Organism:Homo sapiens (human)

            Morphology:fibroblast

            Source:Organ: liver 
            Cell Type: immortalized with SV40 large T antigen

            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
            FL62891細胞， 人肝細胞

            Age:fetus

            Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

            Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
            Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
            Temperature: 32.0°C

            Subculturing:Protocol:
            1. Remove and discard culture medium.
            2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
            3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.
            4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            5. Add appropriate aliquots of the cell suspension to new culture vessels.
            6. Incubate cultures at 32°C.

            Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended 
            Medium Renewal: Every 2 to 3 days

            Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
            Storage temperature: liquid nitrogen vapor phase

            Doubling Time:48 hours

            Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
            recommended serum:ATCC 30-2020


            References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
            70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
            70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
            70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
            70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
            70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















            
						
            

						

						
            
							
																					
            
								
            
									
             
             
            
								
            
								
            
									
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