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            上海復(fù)祥生物科技有限公司
            
			  
			  
            
				
				
			  
            

            




 
 
				
		
            
			
            
				
            
					
            
						
            當(dāng)前位置：上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>人腫瘤細(xì)胞、癌細(xì)胞>>CCL-138Detroit 562細(xì)胞， 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
            
					
            
				
            
			
            
		
            
		
            
			
            
				
            
					
            Detroit 562細(xì)胞， 人咽頭癌胸水轉(zhuǎn)移細(xì)胞 
            
					
            
																								 
						
						  
					
            
					
					
            
                        						    
            
							
															
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            產(chǎn)品型號(hào)：CCL-138
            
							
            品       牌：其他品牌
            
                            
            廠商性質(zhì)：生產(chǎn)商
            
                            							
            所  在  地：上海市
            
						
            
						
						
						
            
                        							
            更新時(shí)間：2024-11-16 17:02:40瀏覽次數(shù)：1688
            
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                                    供貨周期
                  一周
                                                                            規(guī)格
                  T25
                                  
            
                                                                      
            
                                    貨號(hào)
                  Detroit 562
                                                                            應(yīng)用領(lǐng)域
                  醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
                                  
            
                                                                      
            
                                    主要用途
                  科學(xué)研究
                                                
            
        
            
						
            
Detroit 562細(xì)胞， 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
            ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
            
						
						
            
							
            Detroit 562細(xì)胞， 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
            OrganismHomo sapiens, human
            Tissuepharynx: derived from metastatic site: pleural effusion
            Product Formatfrozen
            Morphologyepithelial
            Culture Propertiesadherent
            Biosafety Level1
            Diseasepharyngeal carcinoma
            Age*****
            Genderfemale
            EthnicityCaucasian
            Storage Conditionsliquid nitrogen vapor phase
              Karyotype   Modal number = 64; range = 58 to128
            A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  
            Detroit 562細(xì)胞， 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
            Caucasian
            female
              Genes Expressed  
            keratin
              Virus Susceptibility   Human poliovirus 1 
            Vesicular stomatitis virus              
              Comments  
            The cells are positive for keratin by immunoperoxidase staining.
            Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
             
            Subculturing




            Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
            1. Remove and discard culture medium.
            2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
            3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
            4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
            5. Add appropriate aliquots of the cell suspension to new culture vessels.
            6. Incubate cultures at 37°C.

            Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
            Medium Renewal: Every 2 to 3 days
            Cryopreservation
            Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
            Storage Temperature: liquid nitrogen vapor phase
            Culture Conditions
            Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
            Temperature: 37°C              CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞






















            
						
            

						

						
            
							
																					
            
								
            
									
             
             
            
								
            
								
            
									
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