狠狠色丁香久久综合婷婷亚洲成人福利在线-欧美日韩在线观看免费-国产99久久久久久免费看-国产欧美在线一区二区三区-欧美精品一区二区三区免费观看-国内精品99亚洲免费高清

            上海復(fù)祥生物科技有限公司

            當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫(kù)>>小鼠正常細(xì)胞>>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

            MS1 小鼠胰島內(nèi)皮細(xì)胞

            參   考   價(jià):面議
            具體成交價(jià)以合同協(xié)議為準(zhǔn)

            產(chǎn)品型號(hào):CRL-2279

            品       牌:ATCC

            廠商性質(zhì):生產(chǎn)商

            所  在  地:上海市

            更新時(shí)間:2025-04-26 21:18:27瀏覽次數(shù):2389

            聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)
            供貨周期 一周 規(guī)格 T25
            貨號(hào) MS1 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
            主要用途 科學(xué)研究
            CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

            CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

            CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

            ATCC® Number:  CRL-2279™

            Designations:  MS1 (MILE SVEN 1)

            Depositors:   JL Arbiser

            Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

            Shipped:  froz


            Organism: Mus musculus (mouse)en

            Medium & Serum:  See Propagation

            Growth Properties: adherent

            Morphology: endothelial


            CRL-2279

            Source: Organ: pancreas

            Strain: C57BL/6

            Tissue: islet of Langerhans; endothelium

            Cell Type: SV40 transformed

            Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

            Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


            Receptors: vascular endothelial growth factor (VEGF), expressed

            Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

            Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

            Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

            The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

            The cells are useful for the study of signal transduction of angigenic factors.

            It is the only line known that can give rise to benign hemangiomas.

            Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

            Atmosphere: air, 95%; carbon dioxide (CO2), 5%

            Temperature: 37.0°C

            Subculturing:  Protocol:

            Remove and discard culture medium.

            Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

            Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

            Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

            Add appropriate aliquots of the cell suspension to new culture vessels.

            Incubate cultures at 37°C.

            Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

            Medium Renewal: 2 to 3 times per week

            Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

            Storage temperature: liquid nitrogen vapor phase

            Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

            recommended serum:ATCC 30-2020

            References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

            51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















            NCTC 1469 小鼠正常肝

            CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞

            HT22 小鼠海馬神經(jīng)元細(xì)胞系

            HT22 小鼠海馬神經(jīng)元細(xì)胞系$r$n上海復(fù)祥生物

            TC-1 小鼠肺上皮細(xì)胞

            TC-1 小鼠肺上皮細(xì)胞$r$n上海復(fù)祥生物提供

            會(huì)員登錄

            X

            請(qǐng)輸入賬號(hào)

            請(qǐng)輸入密碼

            =

            請(qǐng)輸驗(yàn)證碼

            收藏該商鋪

            X
            該信息已收藏!
            標(biāo)簽:
            保存成功

            (空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

            常用:

            提示

            X
            您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

            以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

            溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

            撥打電話
            在線留言