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            當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫(kù)>>小鼠腫瘤細(xì)胞、癌細(xì)胞>>TIB-67J774A.1 小鼠腹水單核細(xì)胞瘤

            J774A.1 小鼠腹水單核細(xì)胞瘤

            參   考   價(jià):面議
            具體成交價(jià)以合同協(xié)議為準(zhǔn)

            產(chǎn)品型號(hào):TIB-67

            品       牌:ATCC

            廠商性質(zhì):生產(chǎn)商

            所  在  地:上海市

            更新時(shí)間:2024-11-14 21:00:36瀏覽次數(shù):2373

            聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)
            供貨周期 一周 規(guī)格 T25
            貨號(hào) J774A.1 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
            主要用途 科學(xué)研究
            TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

            TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

            TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤

            ATCC® Number:TIB-67™  Price:$256.00
            Designations:J774A.1

            Depositors:P Ralph

            Biosafety Level:1

            Shipped:frozen

            Medium & Serum:See Propagation

            Growth Properties:adherent

            Organism:Mus musculus (mouse)

            Morphology:macrophage
            J774A.1 小鼠腹水單核細(xì)胞瘤


            Source:Tissue: ascites
            Strain: BALB/cN
            Disease: reticulum cell sarcoma
            Cell Type: monocyte/macrophage macrophage;


            Cellular Products:interleukin 1 beta

            lysozyme [1080]



            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
            TIB-67

            Isolation:La Jolla California, United States
            Isolation date: 1968


            Applications:Biological response [92560]

            transfection host (Roche FuGENE® Transfection Reagents)



            Receptors:complement (C3), expressed [1135]

            Fc receptor, IgG, high affinity I (Fcgr1), expressed [13710]



            Age:adult

            Gender:female

            Comments:J774A.1 cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS [Pubmed: 318922]. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

            Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
            Atmosphere: air, 95%; carbon dioxide (CO2), 5%
            Temperature: 37.0°C


            Subculturing:Protocol: Subcultures are prepared by scraping.

            For a 75 cm2 flask, remove all but 10 ml of the culture medium. (adjust volume accordingly for different culture vessels) Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.


            Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
            Medium Renewal: Replace or add medium two or three times weekly


            Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
            Storage temperature: liquid nitrogen vapor phase


            Doubling Time:17 hours

            Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

            recommended serum:ATCC 30-2020

            purified RNA:ATCC TIB-67R



            References:1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

            1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

            1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

            13710: Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

            22827: Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721

            22896: Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071

            32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

            32883: Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487

            92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

















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