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            上海復(fù)祥生物科技有限公司

            當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>小鼠腫瘤細(xì)胞、癌細(xì)胞>>CRL-1708QT6 鵪鶉?yán)w維肉瘤細(xì)胞

            QT6 鵪鶉?yán)w維肉瘤細(xì)胞

            參   考   價(jià): ¥ 1

            訂  貨  量: ≥1 件

            具體成交價(jià)以合同協(xié)議為準(zhǔn)

            產(chǎn)品型號:CRL-1708

            品       牌:ATCC

            廠商性質(zhì):生產(chǎn)商

            所  在  地:上海市

            更新時(shí)間:2024-11-14 22:29:46瀏覽次數(shù):2668

            聯(lián)系我時(shí),請告知來自 化工儀器網(wǎng)
            供貨周期 一周 規(guī)格 T25
            貨號 QT6 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
            主要用途 科學(xué)研究
            CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

            CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞的詳細(xì)介紹

            CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞

            ATCC® Number:CRL-1708™  Price:$438.00
            Designations:QT6

            Depositors:C Moscovici

            Biosafety Level:1

            Shipped:frozen

            Medium & Serum:See Propagation

            Growth Properties:adherent

            Organism:Coturnix coturnix japonica (quail, Japanese)

            Morphology:fibroblast


            Source:Disease: fibrosarcoma
            Cell Type: methylcholanthrene induced


            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



            Applications:transfection host (Roche FuGENE® Transfection Reagents)

            Virus Susceptibility:Rous sarcoma virus

            Avian encephalomyelitis virus

            Rabies virus

            Marek's disease virus



            Tumorigenic:Yes

            Propagation:ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
            Temperature: 37.0°C


            Subculturing:Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximay 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
            Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
            Medium Renewal: Twice per week


            Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
            Storage temperature: liquid nitrogen vapor phase


            Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

            recommended serum:ATCC 30-2030



            References:22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

            CRL-1708


















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