狠狠色丁香久久综合婷婷亚洲成人福利在线-欧美日韩在线观看免费-国产99久久久久久免费看-国产欧美在线一区二区三区-欧美精品一区二区三区免费观看-国内精品99亚洲免费高清

            上海復(fù)祥生物科技有限公司

            當(dāng)前位置:> 供求商機(jī)> HTB-38 HT-29 人結(jié)腸癌細(xì)胞

            [供應(yīng)]HTB-38 HT-29 人結(jié)腸癌細(xì)胞

            貨物所在地:上海上海市

            更新時(shí)間:2025-03-21 14:10:01

            有效期:2025年3月21日 -- 2025年9月18日

            已獲點(diǎn)擊:520

            聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)

            低價(jià)供應(yīng) HTB-38 HT-29 人結(jié)腸癌細(xì)胞 ATCC 細(xì)胞 細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

            HTB-38 HT-29 人結(jié)腸癌細(xì)胞 

            低價(jià)供應(yīng) HTB-38 HT-29 人結(jié)腸癌細(xì)胞
            ATCC? Number:  HTB-38?       
            Designations:  HT-29  
            Depositors:   J Fogh  
            Biosafety Level: 1  
            Shipped:  frozen  
            Medium & Serum:  See Propagation  
            Growth Properties: adherent 
            Organism: Homo sapiens (human)  
            Morphology: epithelial

             
            Source: Organ: colon 
            Disease: colorectal adenocarcinoma 
            Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin  
            Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
             
            Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.  
            Isolation:  Isolation date: 1964  低價(jià)供應(yīng)
            Applications: transfection host (Nucleofection technology from Lonza
            Roche FuGENE? Transfection Reagents) 
            Receptors: human adrenergic alpha2A [23560] 
            urokinase receptor (u-PAR) 
            vitamin D (moderate expression) 
            urokinase receptor (u-PAR); vitamin D (moderate expression) 
            Virus Susceptibility: human immunodeficiency virus (HIV, LAV) 
            Tumorigenic: Yes  
            Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -  
            Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5 
            DNA Profile (STR): Amelogenin: X 
            CSF1PO: 11,12 
            D13S317: 11,12 
            D16S539: 11,12 
            D5S818: 11,12 
            D7S820: 10 
            THO1: 6,9 
            TPOX: 8,9 
            vWA: 17,19 
            Cytogenetic Analysis: modal number = 71; range = 68 to 72.
            The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis. 
            Isoenzymes:  AK-1, 1 
            ES-D, 1 
            G6PD, B 
            GLO-I, 1-2 
            Me-2, 1   
            PGM1, 1-2 
            PGM3, 1-2 
            Age:  44 years adult  
            Gender:  female  
            Ethnicity:  Caucasian  
            Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected. 
            There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. 
            Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
            Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
            Temperature: 37.0°C 
            Subculturing:  Protocol: 
            Remove and discard culture medium.
            Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
            Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
            Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            Add appropriate aliquots of the cell suspension to new culture vessels.
            Incubate cultures at 37C.

            Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended 
            Medium Renewal: 2 to 3 times per week 
            Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% 
            Storage temperature: liquid nitrogen vapor temperature 
            Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
            recommended serum:ATCC 30-2020
            derivative:ATCC CCL-218
            purified DNA:ATCC HTB-38D 

            MLE-12 小鼠肺上皮細(xì)胞   
            saos-2 人成骨肉瘤細(xì)胞
            MA104 非洲綠猴胚胎腎上皮細(xì)胞
            ball-1 細(xì)胞
            nci-h226 人肺鱗癌細(xì)胞
            MCA38 小鼠結(jié)腸癌細(xì)胞
            LTPA 小鼠胰腺癌細(xì)胞 
            NCI-H23 人非小細(xì)胞肺癌細(xì)胞
            293.00     人胚腎細(xì)胞

            低價(jià)供應(yīng)
            293FT    人胚腎細(xì)胞-用于慢病毒包裝
            293TN    人胚腎細(xì)胞--用于慢病毒包裝
            293AV    人胚腎細(xì)胞-用于腺病毒包裝
            293T    人胚腎細(xì)胞
            208F    大鼠成纖維細(xì)胞
            786-O    人腎透明細(xì)胞腺癌細(xì)胞
            A172    人腦膠質(zhì)母細(xì)胞瘤細(xì)胞
            A549    人肺癌細(xì)胞 細(xì)胞系購買
            AGS    人胃癌細(xì)胞
            BEL7402 人肝癌細(xì)胞系
            AZ-521    人胃癌細(xì)胞
            B16F0    小鼠黑色素瘤細(xì)胞
            Bel-7402    人肝癌細(xì)胞
            Bel-7404    人肝癌細(xì)胞
            BGC-823    人胃癌細(xì)胞
            C6    大鼠腦膠質(zhì)瘤細(xì)胞
            SW480    人結(jié)腸腺癌細(xì)胞
            CaCo-2    人結(jié)腸癌細(xì)胞
            Cho-K1    倉鼠卵巢細(xì)胞亞株
            COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
            GES-1    人胃上皮細(xì)胞
            HCT-8    人回盲腸癌細(xì)胞
            HeLa    人宮頸癌細(xì)胞
            Hepa1-6    小鼠肝癌細(xì)胞
            HepG2    人肝癌細(xì)胞
            hFOB1.19    人永生化成骨細(xì)胞
            HGC-27    人胃癌細(xì)胞
            Hs 746T    人胃癌細(xì)胞
            HT-29    人結(jié)腸癌細(xì)胞
            KB    人口腔表皮樣癌細(xì)胞
            LoVo    人結(jié)腸癌細(xì)胞
            LLC-PK1    豬腎近曲小管上皮細(xì)胞
            MCF-10A    人正常乳腺上皮細(xì)胞
            MCF-7    人乳腺癌細(xì)胞
            MDA-MB-231    人乳腺癌細(xì)胞
            MGC80-3    人胃癌細(xì)胞
            MKN-45    人胃癌細(xì)胞

              
            NCI-H460    人大細(xì)胞肺癌細(xì)胞
            NIH-3T3    小鼠成纖維細(xì)胞
            NUGC-3    人胃癌細(xì)胞
            Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞
            SGC-7901    人胃癌細(xì)胞
            SMMC-7721    人肝癌細(xì)胞
            SNU-638    人胃癌細(xì)胞
            SNU-739    人肝癌細(xì)胞
            Tca8113    人舌鱗癌細(xì)胞    細(xì)胞系購買
            U251    人腦膠質(zhì)瘤細(xì)胞
            U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
            U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤
            BT-474    人乳腺導(dǎo)管癌細(xì)胞
            PANC-1    人胰腺癌細(xì)胞
            HEK293    人胚腎細(xì)胞
            Hep3B    人肝癌細(xì)胞
            HL-7702    人正常肝細(xì)胞
            Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞
            WEHI-231    小鼠B淋巴細(xì)胞
            OPM-2    人骨髓瘤細(xì)胞
            NCI-H929    人骨髓瘤細(xì)胞

            低價(jià)供應(yīng)
            U266    人骨髓瘤細(xì)胞
            EJ    人膀胱癌細(xì)胞
            HOC    大鼠肝卵圓細(xì)胞
            RWPE-1    人正常前列腺上皮細(xì)胞
            CAL 27    人舌鱗癌細(xì)胞
            Calu-3    人肺腺癌細(xì)胞
            Eca-109    人食管癌細(xì)胞
            DLD-1    人結(jié)直腸腺癌上皮細(xì)胞
            5637.00     人膀胱癌細(xì)胞
            SK-OV-3    人卵巢癌細(xì)胞
            GBC-SD    人膽囊癌細(xì)胞
            BEL7402 人肝癌細(xì)胞系
            SW620    人結(jié)腸癌細(xì)胞
            MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購買
            PC9    人肺癌細(xì)胞
            PC14    人肺癌細(xì)胞
            BxPC    人原位胰腺腺癌細(xì)胞
            PK(15)    豬腎上皮細(xì)胞
            MDCK    狗腎上皮細(xì)胞
            3D4/21    豬肺泡巨噬細(xì)胞
                
                
                
            MHCC-97L    人肝癌細(xì)胞(低轉(zhuǎn)移)
            MHCC-97H    人肝癌細(xì)胞系(高轉(zhuǎn)移)
            MiaCaPa-2    人胰腺癌細(xì)胞
            HN13    口腔鱗狀細(xì)胞癌細(xì)胞
            Bend3    小鼠腦微血管內(nèi)皮細(xì)胞
            QBC939    人膽管癌細(xì)胞
            Ishikawa    人子宮內(nèi)膜癌細(xì)胞
            A498    人腎癌細(xì)胞
            143B    
            WEHI-231    小鼠B淋巴細(xì)胞
            BN    
            P9R    小鼠骨髓基質(zhì)細(xì)胞
            P9    小鼠骨髓基質(zhì)細(xì)胞
            F98    大鼠腦膠質(zhì)瘤細(xì)胞
            NCIH1299    人非小細(xì)胞肺癌細(xì)胞
            NCIH1650-M3    人非小細(xì)胞肺癌細(xì)胞
            293LVT    人胚腎細(xì)胞--用于慢病毒包裝
            EC9706    人食管癌細(xì)胞
            CNE    人鼻咽癌細(xì)胞
            H322    人非小細(xì)胞肺癌細(xì)胞
            Panc O2    
            S8    BEL7402 人肝癌細(xì)胞系
            R5    細(xì)胞系購買
            Pig MSC    豬骨髓間質(zhì)干細(xì)胞
            HCAEC    人冠狀動(dòng)脈內(nèi)皮細(xì)胞
            marc-145    猴胚胎腎上皮細(xì)胞
            3D4/21    豬肺泡巨噬細(xì)胞
            PK(15)    豬腎上皮細(xì)胞
            MADB-106    大鼠乳腺癌細(xì)胞
            Sf9    昆蟲卵巢細(xì)胞
            DADY    
            HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞
            PC14    人肺癌細(xì)胞

            低價(jià)供應(yīng)
            PC9    人肺癌細(xì)胞
            Eca-109    人食管癌細(xì)胞
            CAL 27    人舌鱗癌細(xì)胞
            HOC    肝卵圓細(xì)胞
            EJ    人膀胱癌細(xì)胞

            HGC-27 人胃癌細(xì)胞(未分化
            U266    人骨髓瘤細(xì)胞
            293-mTLR5    人胚腎細(xì)胞
            Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞
            HL-7702    人正常肝細(xì)胞
            Hep3B    人肝癌細(xì)胞
            U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
            Tca-8113    人舌鱗癌細(xì)胞
            SNU-739    人肝癌細(xì)胞
            SNU-638    人胃癌細(xì)胞
            NUGC-3    人胃癌細(xì)胞
            NIH-3T3    小鼠成纖維細(xì)胞
            MKN-45    人胃癌細(xì)胞
            LLC-PK1    豬腎近曲小管上皮細(xì)胞
            Hs 746T    人胃癌細(xì)胞
            Hepa1-6    小鼠肝癌細(xì)胞
            HCT-8    人回盲腸癌細(xì)胞
            BEL7402 人肝癌細(xì)胞系
            HaCaT    人永生化表皮細(xì)胞
            GES-1    人胃上皮細(xì)胞
            B16-F0    小鼠黑色素瘤細(xì)胞
            AZ-521    人胃癌細(xì)胞
            786-O    人腎透明細(xì)胞腺癌細(xì)胞
            208F    大鼠成纖維細(xì)胞
            293TN    人胚腎細(xì)胞--用于慢病毒包裝
            293FT    人胚腎細(xì)胞-用于慢病毒包裝
            GP-293    人胚腎細(xì)胞
            293-L.P.    人胚腎細(xì)胞 

            會(huì)員登錄

            X

            請(qǐng)輸入賬號(hào)

            請(qǐng)輸入密碼

            =

            請(qǐng)輸驗(yàn)證碼

            收藏該商鋪

            X
            該信息已收藏!
            標(biāo)簽:
            保存成功

            (空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

            常用:

            提示

            X
            您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

            以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

            溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

            撥打電話
            在線留言