MA104 非洲綠猴胚胎腎上皮細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
MA104 非洲綠猴胚胎腎上皮細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
MA104 非洲綠猴胚胎腎上皮細(xì)胞 的詳細(xì)介紹
MA104 非洲綠猴胚胎腎上皮細(xì)胞
| ATCC? Number: | CRL-2378.1? | Price: | $365.00 |
| Designations: | MA-104 Clone 1 | | Depositors: | MM Vincent | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Cercopithecus aethiops | | Morphology: | epithelial
| | Source: | Organ: kidney
Cell Type: epithelial | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Isolation: | Manassas Virginia, United States
Isolation date: 2001 | | Applications: | transfection host （Roche FuGENE? Transfection Reagents） | | Virus Susceptibility: | Simian rotavirus | | Age: | embryo | | Comments: | This item is not available for international distribution. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: 1:3 to 1:10
Medium Renewal: Every 2 to 3 days | | Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase | | Doubling Time: | 27 hours | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2378 | | References: | 38895: Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530 |
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