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            [供應(yīng)]CCL-25-WISH 人羊膜細胞

            貨物所在地:上海上海市

            產(chǎn)地:美國

            更新時間:2025-04-02 09:28:22

            有效期:2025年4月2日 -- 2026年4月2日

            已獲點擊:115

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            CCL-25 WISH 人羊膜細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

            CCL-25 WISH 人羊膜細胞

            ATCC® Number:CCL-25™    Price:$323.00
            Additional information about this cell line
            Designations:WISH
            Depositors: L Hayflick
            Biosafety Level:2
            Shipped:frozen
            Medium & Serum:See Propagation
            Growth Properties:adherent
            Organism:Homo sapiens (human)
            Morphology:epithelial
             
            Source:Organ: HeLa contaminant
            Cellular Products:keratin
            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
             
            Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)
            Reverse Transcript:negative
            DNA Profile (STR):Amelogenin: X 
            CSF1PO: 9,10 
            D13S317: 13.3 
            D16S539: 9,10 
            D5S818: 11,12 
            D7S820: 8,12 
            THO1: 7 
            TPOX: 8,12 
            vWA: 16,18
            Isoenzymes:G6PD, A
            HeLa Markers:Y
            Comments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. 
            The cells are positive for keratin by immunoperoxidase staining. 
            NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
            Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
            Temperature: 37.0°C
            Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended 
            Medium Renewal: Twice per week 
            Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
            Preservation:culture medium 95%; DMSO, 5%
            Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
            recommended serum:ATCC 30-2020
            References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed:13712490
            26034: . . Am. J. Dis. Child. 103: 460, 1962.

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