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            [供應(yīng)]CCL-153 HFL1-CCL-153 HFL1 人肺成纖維細(xì)胞

            貨物所在地:上海上海市

            產(chǎn)地:美國(guó)

            更新時(shí)間:2025-04-02 09:28:22

            有效期:2025年4月2日 -- 2026年4月2日

            已獲點(diǎn)擊:93

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            CCL-153 HFL1 人肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

            CCL-153 HFL1 人肺成纖維細(xì)胞
            ATCC® Number:  CCL-153™       
            Designations:  HFL1  
            Depositors:   RG Crystal  
            Biosafety Level: 1  
            Shipped:  frozen  
            Medium & Serum:  See Propagation  
            Growth Properties: adherent 
            Organism: Homo sapiens (human)  
            Morphology: fibroblast

             
            Source: Organ: lung 
            Disease: normal 
            Cell Type: fibroblast 
            Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
             
            Reverse Transcript: negative  
            DNA Profile (STR): Amelogenin: X,Y 
            CSF1PO: 10,12 
            D13S317: 11,12 
            D16S539: 9,11 
            D5S818: 12 
            D7S820: 9,10 
            THO1: 7,9 
            TPOX: 6,9 
            vWA: 17 
            Cytogenetic Analysis: normal human; diploid; stable 
            Isoenzymes:  G6PD, B 
            Age:  fetus  
            Ethnicity:  Caucasian  
            Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
            Temperature: 37.0°C 
            Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended 
            Medium Renewal: 2 to 3 times per week 
            Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
            Add fresh culture medium, aspirate and dispense into new culture flasks. 
            Preservation:  culture medium 95%; DMSO, 5% 
            Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
            recommended serum:ATCC 30-2020 
            References: 23400: Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635 
             

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