CCL-56 Pt-K2 袋鼠腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CCL-56 Pt-K2 袋鼠腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CCL-56 Pt-K2 袋鼠腎細(xì)胞 的詳細(xì)介紹
| ATCC® Number: | CCL-56™ | Price: | $407.00 |
| Designations: | PtK2 （NBL-5） | | Depositors: | KH Whalen | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Potorous tridactylis （potoroo） | | Morphology: | epithelial
| | Source: | Organ: kidney
Disease: normal | | Cellular Products: | keratin | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Applications: | transfection host （Roche FuGENE® Transfection Reagents） | | Virus Resistance: | adenovirus 5; coxsackievirus B5; poliovirus 2 | | Age: | adult | | Gender: | male | | Comments: | The cells are positive for keratin by immunoperoxidase staining. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days | | Preservation: | Freeze medium: growth medium, 95%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020 | | References: | 26208: . . Genetics 51: 915, 1965. |
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