| Designations: | MDBK （NBL-1） | | Depositors: | S Madin, NB Darby | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Bos taurus （cow） | | Morphology: | epithelial
| | Source: | Organ: kidney
Disease: normal | | Cellular Products: | keratin MDBK （NBL-1） 牛腎細胞 | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Applications: | detection and quantitation of replication competent retroviruses [92434]
transfection host （Nucleofection technology from Lonza） | | Virus Resistance: | poliovirus 2 | | Cytogenetic Analysis: | Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes （4-5 metacentric, 3-4 submetacentric and 4-5 acro-ocentric） common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen. | | Age: | adult | | Gender: | male | | Comments: | The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby.
It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus （BVDV）.
In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection.
The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule （F-145）. Extensive testing （35 passages） by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK.
The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.
The cells are positive for keratin by immunofluorescence.
The line has been shown to be free of bovine viral diarrhea virus （BVDV）. [22883] |
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