CRL-2285 Super Tube 狗腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CRL-2285 Super Tube 狗腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CRL-2285 Super Tube 狗腎細(xì)胞 的詳細(xì)介紹
| ATCC® Number: | CRL-2285™ | | |
| Designations: | Super Tube | | Depositors: | RJ Klebe | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Canis familiaris | | Morphology: | epithelial
| | Source: | Organ: kidney
Disease: normal | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Age: | adult | | Gender: | female | | Comments: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK （ATCC CCL-34） cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Super tube forms large numbers of tubules when maintained at high cell density （cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate）.
For tubule formation, the cells should be fed twice a day by a complete medium change.
Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome （ATCC CRL- 2286） and MDCK. | | Propagation: | ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10 fetal bovine serum.
Temperature: 37.0°C | | Subculturing: | Protocol: Remove medium, and rinse with 0.25 trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days | | Preservation: | Freeze medium: Complete growth medium 95; DMSO, 5
Storage temperature: liquid nitrogen vapor temperature | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2006
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-34
derived from same cell line:ATCC CRL-2286 | | References: | 38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715 |
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