CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷 的詳細(xì)介紹
ATCC® Number: CRL-9096™
Designations: CHO/dhFr- [CHO duk-, NOTE: dhfr refers to dihydrofolate reductase]
Depositors: Hoffmann-La Roche Ltd.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus （hamster, Chinese）
Morphology: epithelial
CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷
Source: Organ: ovary
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host （Roche FuGENE® Transfection Reagents）
Gender: female
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.1 mM hypoxanthine, 0.016 mM thymidine, 0.002mM Methotrexate （Amethopterin） and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: The cells are deficient in dihydrofolate reductase.They should die in the absence of HT （ Hypoxanthine-Thymidine）. Methotrexate （Amethopterin） is added to the cell culture medium to prevent growth of revertant cells with a low resistance to the drug.
Subculturing: Protocol: CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005
recommended serum:ATCC 30-2020
Medium additive:ATCC 71-X
References: 26539: Shulman T, et al. An antibody reactive with domain 4 of the plaet-derived growth factor beta receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. J. Biol. Chem. 272: 17400-17404, 1997. PubMed: 9211881 CHO/dhFr- 倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷
30382: Morel-Kopp MC, et al. A three amino acid deletion in glycoprotein IIIa is responsible for type I glanzmann's thrombasthenia: importance of residues I1e325Pro326Gly327 for beta 3 integrin subunit association. Blood 90: 669-677, 1997. PubMed: 9226167
30522: Libyh MT, et al. A recombinant human scFv anti-Rh（D） antibody with multiple valences using a C-terminal fragment of C4-binding protein. Blood 90: 3978-3983, 1997. PubMed: 9354666
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