活性氧檢測(cè)試劑盒(Reactive Oxygen Species Assay Kit)（CAT# 50101ES01）是一種基于熒光染料DCFH-DA (2,7-Dichlorodi -hydrofluorescein diacetate)的熒光強(qiáng)度變化，定量檢測(cè)細(xì)胞內(nèi)活性氧水平方法。

DCFH-DA本身沒(méi)有熒光，可以自由穿過(guò)細(xì)胞膜，進(jìn)入細(xì)胞內(nèi)后，可以被細(xì)胞內(nèi)的酯酶水解生成DCFH。而DCFH不能透過(guò)細(xì)胞膜，從而使探針很容易被標(biāo)記聚集在細(xì)胞內(nèi)。細(xì)胞內(nèi)的活性氧能夠氧化無(wú)熒光的DCFH生成有熒光的DCF。DCF綠色熒光強(qiáng)度與細(xì)胞內(nèi)活性氧水平成正比，檢測(cè)DCF的熒光就可以知道細(xì)胞內(nèi)活性氧水平。

在激發(fā)波長(zhǎng)488 nm，發(fā)射波長(zhǎng)525 nm條件下，使用熒光顯微鏡、激光共聚焦顯微鏡、熒光分光光度計(jì)、熒光酶標(biāo)儀、流式細(xì)胞儀等檢測(cè)DCF熒光，從而測(cè)定細(xì)胞內(nèi)活性氧水平。

相關(guān)產(chǎn)品：

"519-37-97-(2-羥基乙基)茶堿7-(2-Hydroxyethyl)theophylline"

"1313403-49-47-BIA"

"1263094-00-37-Chlorokynurenic acid sodium salt "

"26927-01-57-Desacetoxy-6,7-dehydrogedunin"

"162784-72-77-Epi-10-oxo-docetaxel"

"153381-68-17-Epi-docetaxel"

"121569-61-77-epi-Hydroxystaurosporine"

"105454-04-47-Epitaxol,95%"

"1621-56-37-Methoxyisoflavone "

"90332-66-47-xylosyltaxol"

"19037-69-5 7-Hydroxy-4-methyl-8-nitrocoumarin"

"59887-89-77-羥基-4-色酮7-Hydroxy-4-benzopyrone"

"301356-95-679-6 (CID5721353, BCL6 inhibitor)"

"1472624-85-37ACC2 "

"76939-46-38-Bromo-cAMP"

"3868-31-38-Hydroxyguanosine "

"78950-78-48-OH-DPAT (8-Hydroxy-DPAT)"

"1123-54-28-氮雜腺嘌呤8-Azaadenine"

2342583-66-6

9-(1-Cyclopropylethyl)-N2-(2-(dimethylamino)ethyl)-N6-(3-(trifluoromethyl)benzyl)-9H-purine-2,6-diamine

"1639411-87-29-Azido-Neu5DAz "

"91421-43-19-氨基喜樹(shù)堿9-Aminocamptothecin"

"91421-42-09-硝基喜樹(shù)堿9-Nitrocamptothecin"