QQ交談
您所在位置：請輸入產(chǎn)品關(guān)鍵字：
郵編：200431
聯(lián)系人：王小姐
電話：021-56640936
傳真：021-33250231
手機：13122441390 15900755943
留言：發(fā)送留言
個性化：www.shifengsj.com
網(wǎng)址：www.shfeng-edu.com
商鋪：http://apwanrong.com/st236594/
實時定量RT
點擊次數(shù)：570 發(fā)布時間：2016-1-25
I. Assay Development
A. Sequence selection
B. primer & Probe Selection
C. Quencher dye and inteRNAl reference
D. Assay Validation
II. Assay Setup
A. One- vs Two-Step RT-PCR
B. Plate Setups for Robotics
C. thermocycler settings
III. Data Analysis
A. Baseline and threshold settings
B. Standard Curves
C. Inter- vs intra-assay variABIlity
D. Sample normalization
taq Exonuclease Reaction
Sequence Selection
The most important component of building an assay
Make sure the initial sequence is the correct one
Use Blast at the ncbi to find other sequences (http://www.ncbi.nlm.nih.gov/)
Download all the unique sequences you find, including other species (human, mouse, rat)
Look particularly for the NM_ or refseqs