N-（3-二甲基氨基丙基）-N′-乙基碳二亞胺 鹽酸鹽

性質(zhì)

產(chǎn)品描述

包裝

1, 5, 10, 25 g in poly bottle

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
• for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
• for cross-linking of nanofiber matrices with mouse lung extract
• for activation of substrate XNA oligonucleotides
 

Biochem/physiol Actions

水溶性縮合試劑。EDAC通常用作與伯胺進(jìn)行酰胺鍵合的羧基活化劑。另外，它能與磷酸基團(tuán)反應(yīng)。其已用于例如肽合成、將蛋白質(zhì)與核酸交聯(lián)、以及免疫綴合物的制備。通常，EDAC在不含緩沖劑的pH范圍4.0-6.0的條件下使用。特別是，應(yīng)避免使用胺和羧酸鹽緩沖液。