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            RG001060-anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)
            • RG001060-anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)

            貨物所在地:北京北京市

            地: 北京通州

            更新時間:2025-02-13 21:00:07

            瀏覽次數(shù):693

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            ( 聯(lián)系我們,請說明是在 化工儀器網(wǎng) 上看到的信息,謝謝?。?/p>

            anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)在Western Blotting中使用內(nèi)參其實就是在WB過程中的另外用內(nèi)參對應的抗體檢測內(nèi)參,這樣在檢測目的產(chǎn)物的同時可以檢測內(nèi)參的表達,由于內(nèi)參在各組織和細胞中的表達相對恒定,借助檢測每個樣品內(nèi)參的量就可以用于校正上樣誤差,這樣半定量的結(jié)果才更為可信。

            英文名稱      anti-FLAG Tag Rabbit Polyclonal antibody

            應用             WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.

            規(guī)  格           100ul

            濃  度           1mg/ml

            保  存           Store at -20°C. Do not aliquot the antibody.

            儲存液          PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

            純化方法      affinity purified by Protein A

            亞  型           IgG

            性  狀           Liquid

            克隆類型      polyclonal

            宿  主           Rabbit

            The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

            說明書

            Anti- Flag Tag Rabbit Polyclonal Antibody

            Catalog No. RG001060

            Size 100μl

            Storage: Store at -20°C. Do not aliquot the antibody.

            The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to

            interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively

            as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to

            separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can

            also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many

            different assays that require recognition by an antibody. If there is no antibody against the studied protein,

            adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag

            sequence.

            Molecular Weight: N/A

            Application: WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.

            Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag

            fusion proteins.

            Form: Liquid , 1mg/ml

            Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

            Relative Products

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            M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)

            M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)

            M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)

            RG001010 Anti- HA Tag Rabbit Polyclonal Antibody

            RG001020 Anti- His Tag Rabbit Polyclonal Antibody

            RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody

            RG001040 Anti- GST Tag Rabbit Polyclonal Antibody

            RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody

            RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody

            Solarbio


            Beijing Solarbio Science & Technology Co., Ltd

            Http://www.solarbio。。com
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