Recombinant Leukocyte Cell Derived Chemotaxin 1 (LECT1)  

白細(xì)胞衍生趨化因子1(LECT1)重組蛋白

[ PROPERTIES ]

Residues: Lys134~Val334,with two N-terminal Tags, His-tag and T7-tag

Host: E. coli

Subcellular Location: Endomembrane system;Single-pass membrane protein, Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 26.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8oC for one month.

             Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.IPEVGAVTKQSISSKLEGKI MPVKYEENSL IWVAVDQPVK DNSFLSSKVL ELCGDLPIFWLKPTYPKEIQRE R RE V V R KI VP T T T K RP H S GP RS NP G A G R LN N E T RP S V Q EDS Q A F NP D NPYHQQEGESM TFDPRLDHEG ICCIECRRSY THCQKICEPL GGYYPWPYNY QGCRSACRVIMPCSWWVARI LGMV

[  IDENTIFICATION  ]

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