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            [供應(yīng)]Y30055-人源肝臟細(xì)胞及iPS向肝臟細(xì)胞定向分化試劑盒

            貨物所在地:上海上海市
            更新時(shí)間:2024-12-25 17:12:43
            有效期:2024年12月25日 -- 2025年6月26日
            已獲點(diǎn)擊:263

            產(chǎn)品簡(jiǎn)介
            Cellartis iPS Cell to Hepatocyte Differentiation System可以穩(wěn)定地、規(guī)?;貙?shí)現(xiàn)iPS向hepatocyte的誘導(dǎo)轉(zhuǎn)化生產(chǎn),而且誘導(dǎo)轉(zhuǎn)化出的肝細(xì)胞能夠穩(wěn)定表達(dá)藥物代謝相關(guān)酶系統(tǒng)和藥物轉(zhuǎn)運(yùn)系統(tǒng)。
            歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :152 1681 4001。華雅再生醫(yī)學(xué)
            詳細(xì)介紹

            歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

            人源肝臟細(xì)胞及iPS向肝臟細(xì)胞定向分化的解決方案提供者

             

            iPS向肝臟細(xì)胞定向分化操作系統(tǒng)

            (Cellartis iPS Cell to Hepatocyte Differentiation System)

            以肝臟細(xì)胞為模型的實(shí)驗(yàn)越來(lái)越受到重視,在肝臟病變機(jī)理研究、藥物代謝研究、藥物毒理評(píng)估等方面有廣闊的應(yīng)用前景。然而,以個(gè)體原代肝細(xì)胞為材料來(lái)源會(huì)產(chǎn)生如取材困難、材料批間差大、無(wú)法長(zhǎng)期穩(wěn)定供應(yīng)等一系列難以克服的問(wèn)題。因此,通過(guò)iPS批量轉(zhuǎn)化生產(chǎn)肝細(xì)胞的方法就顯示出特別的優(yōu)勢(shì)。Cellartis iPS Cell to Hepatocyte Differentiation System可以穩(wěn)定地、規(guī)?;貙?shí)現(xiàn)iPShepatocyte的誘導(dǎo)轉(zhuǎn)化生產(chǎn),而且誘導(dǎo)轉(zhuǎn)化出的肝細(xì)胞能夠穩(wěn)定表達(dá)藥物代謝相關(guān)酶系統(tǒng)和藥物轉(zhuǎn)運(yùn)系統(tǒng)。

            Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統(tǒng),客戶既可以對(duì)某個(gè)特殊病人來(lái)源的iPS實(shí)現(xiàn)轉(zhuǎn)化,也可以利用cellartis供應(yīng)的iPS進(jìn)行標(biāo)準(zhǔn)化操作。使用本產(chǎn)品的過(guò)程起始于誘導(dǎo)人iPS轉(zhuǎn)化成為確定性內(nèi)胚層(Definitive Endoderm (DE))細(xì)胞,然后誘導(dǎo)轉(zhuǎn)化DE至肝細(xì)胞。本產(chǎn)品是All-In-One型產(chǎn)品,實(shí)驗(yàn)所需的培養(yǎng)基、基質(zhì)(coating)等包括在內(nèi)。應(yīng)用本產(chǎn)品轉(zhuǎn)化約3 × 106 hiPS cells,可以獲得約5 × 106 肝細(xì)胞(大約50 cm2面積的單層貼壁細(xì)胞)。

            利用Cellartis iPS Cell to Hepatocyte Differentiation System轉(zhuǎn)化得到的肝細(xì)胞除了清晰表現(xiàn)出肝細(xì)胞的*特征外(如肝細(xì)胞特異性標(biāo)志物、CYP系列酶等),還保留了原始細(xì)胞供體者的遺傳基因背景。另外,相比較從組織切除獲得的原代肝臟細(xì)胞(通常需要低溫保存),通過(guò)本產(chǎn)品系統(tǒng)轉(zhuǎn)化獲得的肝細(xì)胞在更長(zhǎng)的時(shí)間跨度上保持著生理功能的穩(wěn)定性。這一點(diǎn)對(duì)于毒理學(xué)評(píng)估和病毒感染評(píng)估具有顯著的意義。為長(zhǎng)時(shí)間維持培養(yǎng)hiPS轉(zhuǎn)化而來(lái)的肝細(xì)胞,我們推薦Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051)。

             

            關(guān)于Cellartis iPS Cell to Hepatocyte Differentiation技術(shù)的專項(xiàng)說(shuō)明,咨詢。

            紅榮微再(上海)生物工程技術(shù)有限公司    152 1681 4001    Phone 

             

            Cellartis iPS Cell to Hepatocyte Differentiation System 產(chǎn)品特點(diǎn)

            · Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS

               cell lines, so no need to optimize for your lines.

            · Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS

               cell-derived hepatocytes with diverse genetic backgrounds.

            · Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver

               disease models.

            · Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

            · Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

            歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

            產(chǎn)品成分

            · Cellartis DEF-CS 100 Culture System (Y30020, not sold separay)

            · Cellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separay)

            · Cellartis Hepatocyte Differentiation Kit (Y30050)

             

            Code No. Product    Size

            Y30055     Cellartis® iPS Cell to Hepatocyte Differentiation System        1 Kit

            Y30051     Cellartis® Hepatocyte Maintenance Medium          100 ml

             

            產(chǎn)品實(shí)驗(yàn)圖:

            Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

             

            Cellartis iPS Cell to Hepatocyte Differentiation System 產(chǎn)品訂購(gòu)清單

            Y30050     Cellartis® Hepatocyte Differentiation Kit                          

            Y30051     Cellartis® Hepatocyte Maintenance Medium                           

            Y30035     Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™                            

            組份-1      Cellartis DEF-CS 100 Culture System (Cat. No. Y30020                    

            組份-2      Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030                          

            Y30055     Cellartis DEF-CS 100 Culture System (Cat. No. Y30020,                  

                     Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030                          

                     Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050                       

             歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348                                   

            "Cat. No. Y30020 Package Contents

            Package 1:

            · 100 ml DEF-CS 100 Basal Medium

            · 800 μl DEF-CS COAT-1

            Package 2:

            · 300 μl DEF-CS GF-1

            · 100 μl DEF-CS GF-2

            · 40 μl DEF-CS GF-3

             

            Cat. No. Y30030 Package Contents

            ? 10 ml Definitive Endoderm Differentiation Coating

            ? 18 ml Definitive Endoderm Differentiation Day 0

            ? 18 ml Definitive Endoderm Differentiation Day 1

            ? 18 ml Definitive Endoderm Differentiation Day 2

            ? 25 ml Definitive Endoderm Differentiation Day 3

            ? 47 ml Definitive Endoderm Differentiation Day 4

            ? 47 ml Definitive Endoderm Differentiation Day 6

             

            Cat. No. Y30050 Package Contents

            ? 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

            ? 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

            ? 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

            ? 1 bottle Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

            ? 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

            ? 1 tube Hepatocyte Coating (7.5 ml)"                            

             

            歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

            參考文獻(xiàn):

            Asplund, Annika, et al.One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev and Re

             


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