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            深圳欣博盛生物科技有限公司
            中級會員 | 第5年

            13129540117

            Epicypher熱銷產(chǎn)品—CUTANA™ E. coli Spike-in DNA

            時間:2023/12/14閱讀:427
            分享:

            來源于Escherichia coli(E.coli)的片段DNA可以用作核酸酶靶向切割和釋放(CUT&RUN)的實(shí)驗(yàn)標(biāo)準(zhǔn)化的spike-in對照。產(chǎn)品CUTANA™ E. coli Spike-in DNA含有足夠的材料,可用于100-200個CUT&RUN樣本(高豐度和低豐度靶標(biāo)的范圍)。


            產(chǎn)品詳情

            保存溫度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

            運(yùn)輸溫度: Room temp. Can go on frozen cold packs or dry ice.

            產(chǎn)品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.


            如需購買Epicypher產(chǎn)品,或咨詢產(chǎn)品技術(shù)問題,請聯(lián)系代理商欣博盛生物


            驗(yàn)證數(shù)據(jù)

            Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

            Figure 1: DNA gel data

            E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E. 

            coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in 

            DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions 

            of DNA molecular weight markers are indicated.


            Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

            Figure 2: CUT&RUN sequencing data

            CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched 

            for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher 

            13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads 

            aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE: 

            Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 - 

            5%) of total sequencing reads.


            訂購詳情

            貨號

            產(chǎn)品名稱

            規(guī)格

            18-1401

            CUTANA™ E. coli Spike-in DNA

            100 ng



            Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

            如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品,請聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物 


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