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            當(dāng)前位置：> 供求商機> Murine B16 melanoma - STING Knockout IRF-reporter cells
            
		
            
	
            

            

            
	
            
		
            
			
            [供應(yīng)]Murine B16 melanoma - STING Knockout IRF-reporter cells 
            
			
            
				
						  
			
            
			
            

				
            

														
            貨物所在地：北京北京市
            
                                                        							
            更新時間：2025-04-09 09:30:42
            
							
            有效期：2025年4月9日 -- 2026年4月10日
            
							
            已獲點擊：38
            
				
            
				
				
				
            
					
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						B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway
					
            
					
					
            
						
            Product Name：Murine B16 melanoma - STING Knockout IRF-reporter cells
            Cat#bb-kostg
            B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.
            B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16- F1 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
            B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.
            Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.

            
					
            
                                                                				
				
            
			
            
		
            
	
            


     
    
	  				
							
    
    
    
    
    
    
    
	    
   	
    




        
        




    

    

    

	 
    
    				
			

		
    
    
    
    
    
    
    
    
    
    

    

     
     
     
	 



		 


            
	
            
		
            
			
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