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Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨 F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cell
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Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨
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F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cells. You just need your GFP-bait and RFP-prey plasmids ready for transfection.
F2H® principle
F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus
GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells
GFP-bait is recruited to the platform → bright green spot
If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence
Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨
Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨
F2H® is easy
You get the F2H® cell line
You get the Platform Reagent
You have your GFP-bait and RFP-prey plasmids
Day 1: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H® cells
Day 2: Image and quantify your results