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            蘇州螞蟻淘生物科技有限公司>供求商機>thermo二抗A21203|Invitrogen抗體*
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            • thermo二抗A21203|Invitrogen抗體*

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            貨物所在地: 江蘇蘇州市
            更新時間: 2025-01-31 21:00:07
            期: 2025年1月31日--2025年7月31日
            已獲點擊: 1299
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            產(chǎn)品簡介

            thermo二抗A21203|Invitrogen抗體*
            Alexa Fluor染料是目前較值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料,具有理想的激發(fā),適用于594 nm激光線。

            詳細(xì)介紹

            thermo二抗A21203|Invitrogen抗體*

             

            Alexa Fluor染料是目前值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料,具有理想的激發(fā),適用于594 nm激光線。為了在成像和流式細(xì)胞儀中產(chǎn)生穩(wěn)定的信號,Alexa Fluor 594染料在很寬的摩爾范圍內(nèi)對pH不敏感。具有高熒光量子產(chǎn)率和高光穩(wěn)定性的探針允許以高靈敏度檢測低豐度生物結(jié)構(gòu)。 Alexa Fluor 594染料分子可以高摩爾比附著在蛋白質(zhì)上而不會發(fā)生明顯的自猝滅,從而實現(xiàn)更明亮的結(jié)合物和更靈敏的檢測。每種綴合物的標(biāo)記程度通常為每個IgG分子2-8個熒光團(tuán)分子;每個產(chǎn)品批次的分析證書上都標(biāo)明了確切的標(biāo)簽程度。

            這些驢抗小鼠IgG(H + L)全二級抗體已經(jīng)過親和純化,并顯示出與牛,雞,山羊,豚鼠,倉鼠,馬,人,兔,大鼠和綿羊血清蛋白的小交叉反應(yīng)性。交叉吸附或預(yù)吸附是提高抗體特異性的純化步驟,導(dǎo)致更高的靈敏度和更少的背景染色。使第二抗體溶液通過含有來自潛在交叉反應(yīng)性物質(zhì)的固定化血清蛋白的柱基質(zhì)。在柱中僅捕獲非特異性結(jié)合的二抗,并且高度特異性的第二抗體流過。這種額外步驟的益處在多重/多色染色實驗(例如,流式細(xì)胞術(shù))中是顯而易見的,其中存在與其他一抗的潛在交叉反應(yīng)性或在可能存在內(nèi)源免疫球蛋白的組織/細(xì)胞熒光染色實驗中。

             

            thermo二抗A21203|Invitrogen抗體*

             

             

            Product Details

            Tested Applications

            Dilution

            Immunocytochemistry (ICC)

            4 µg/mL

            Immunofluorescence (IF)

            4 µg/mL

            Immunohistochemistry (IHC)

            1-10 µg/mL

            Published Applications

            Immunohistochemistry (IHC)

            See 5 publications below

            Immunocytochemistry (ICC)

            See 6 publications below

            Immunohistochemistry (Frozen) (IHC (F))

            See 3 publications below

            Miscellaneous PubMed (MISC)

            See 14 publications below

            Product Specifications

            Species Reactivity

            Mouse

            Host / Isotype

            Donkey / IgG

            Class

            Polyclonal

            Type

            Secondary Antibody

            Immunogen

            Gamma Immunoglobins Heavy and Light chains

            Conjugate

            Alexa Fluor® 594

            Excitation/Emission Profile

            View spectra 

            Form

            Liquid

            Concentration

            2 mg/mL

            Purification

            purified

            Storage buffer

            PBS, pH 7.5

            Contains

            5mM sodium azide

            Storage conditions

            4° C, store in dark

            RRID

            AB_2535789

            Target

            IgG

            Antibody Form

            Whole Antibody

            Target Information

             

            We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.

            Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.

            Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

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