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            • Jackson二抗|315-036-046*

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            貨物所在地: 江蘇蘇州市
            更新時(shí)間: 2025-02-13 21:00:07
            期: 2025年2月13日--2025年8月13日
            已獲點(diǎn)擊: 763
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            Jackson二抗|315-036-046*
            通過胃蛋白酶消化全I(xiàn)gG抗體產(chǎn)生F(ab')2片段抗體,以除去大部分Fc區(qū),同時(shí)留下一些鉸鏈區(qū)。

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            Jackson二抗|315-036-046*

            通過胃蛋白酶消化全I(xiàn)gG抗體產(chǎn)生F(ab')2片段抗體,以除去大部分Fc區(qū),同時(shí)留下一些鉸鏈區(qū)。 F(ab')2片段具有通過二硫鍵連接在一起的兩個(gè)抗原結(jié)合Fab部分,因此它們是二價(jià)的。 平均分子量約為110kDa。 它們用于特定應(yīng)用,例如避免二抗與活體細(xì)胞結(jié)合Fc受體或蛋白A或蛋白G。

            基于免疫電泳和/或ELISA,抗體與小鼠IgG重鏈的Fc部分反應(yīng)但不與小鼠免疫球蛋白的Fab部分反應(yīng)。 未檢測到針對(duì)小鼠IgM或非免疫球蛋白血清蛋白的抗體。 已通過ELISA和/或固相吸附測試抗體以確保與人血清蛋白的小交叉反應(yīng),但其可與來自其他物種的免疫球蛋白交叉反應(yīng)。

            Jackson二抗|315-036-046*

             

            Physical State: Freeze-dried solid 
            Storage:

            Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
            Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. 
            Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

             

            Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
            Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
            Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
            Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) 

            Suggested Working Concentration or Dilution Range:

            1:500 - 1:5,000 for immunohisto/cytochemistry
            1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
            1:10,000 - 1:200,000 for Western blotting with ECL substrates

            Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

             

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