CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.

AppearanceLight pink to orange, homogeneous, free flowing powder

SolubilityYellow to orange, clear, complete

pH3.4 ± 0.5

Endotoxin≤1EU/ml

Directions per LiterDissolve 9.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and StabilityStore powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

CMRL培養(yǎng)基富含核酸和維生素，最早被開(kāi)發(fā)用來(lái)培養(yǎng)猴子的腎細(xì)胞和L-cell，適用于人和猴及其他哺乳動(dòng)物細(xì)胞的培養(yǎng)。

接下來(lái)咱們一起看看艾美捷CMRL-1066培養(yǎng)基相關(guān)參數(shù)說(shuō)明~

艾美捷CMRL-1066培養(yǎng)基基本參數(shù)：

英文名稱：C5900-01 CMRL1066 w/o L-Glutamine (Powder)

外觀：淡粉色至橙色，均勻，自由流動(dòng)的粉末

溶解度：黃色至橙色，清澈，完-全

pH：3.4±0.5

內(nèi)毒素：≤1單位/毫升

每升說(shuō)明：將9.7g溶解在800-900ml ddH2O中，輕輕攪拌直至完-全溶解。攪拌時(shí)加入2.2g碳酸氫鈉。將培養(yǎng)基的pH值調(diào)整至低于所需水平的0.1-0.3 pH單位。添加額外的水，使溶液進(jìn)入1L過(guò)濾器，使用0.22微米的膜過(guò)濾器進(jìn)行消毒。等分到無(wú)菌容器中。不要高壓釜。含有不穩(wěn)定的化合物，可能會(huì)因高壓滅菌而損壞。

儲(chǔ)存和穩(wěn)定性：在室溫下儲(chǔ)存粉狀介質(zhì)。收到后可穩(wěn)定12個(gè)月。打開(kāi)的瓶子應(yīng)蓋緊蓋子，并保持在黑暗、低濕度的環(huán)境中。準(zhǔn)備好的培養(yǎng)基應(yīng)保持在4°C，并在短時(shí)間內(nèi)使用。

艾美捷CMRL-1066培養(yǎng)基配方：

無(wú)機(jī)鹽：

Calcium Chloride, anhydrous 0.20

Magnesium Sulfate, anhydrous 0.09769

Potassium Chloride 0.40

Sodium Acetate, anhy 0.05

Sodium Phosphate Monobasic, anhy 0.122

Sodium Chloride 6.80

氨基酸：

L-Alanine 0.025

L-Arginine 0.05787

L-Aspartic Acid 0.03

L-Cysteine•HCl•H2O 0.26

L-Cystine 0.02

L-GlutamicAcid, Sodium 0.0862

L-Glutamine Absent

Glycine 0.05

L-Histidine•HCl•H2O 0.02

trans-4-Hydroxy-L-Proline 0.01

L-Isoleucine 0.02

L-Leucine 0.06

L-Lysine•HCl 0.07

L-Methionine 0.015

L-Phenylalanine 0.025

L-Proline 0.04

L-Serine 0.025

L-Threonine 0.03

L-Tryptophan 0.01

L-Tyrosine 0.04

L-Valine 0.025

維生素：

L-Ascorbic Acid•Na 0.05

PABA 0.00005

D-Biotin 0.00001

Choline Chloride 0.0005

Coenzyme A•Na 0.0025

Cocarboxylase 0.001

2'-Deoxyadenosine 0.01

2'-Deoxycytidine•HCl 0.0116

2'-Deoxyguanosine 0.01

Folic Acid 0.00001

myo-Inositol 0.00005

5-Methyldeoxycytidine 0.0001

?-NAD 0.007

?-NADP•Na 0.001

Nicotinic Acid 0.000025

Niacinamide 0.000025

D-PantothenicAcid•Ca 0.00001

Pyridoxal•HCl 0.000025

Pyridoxine•HCl 0.000025

Riboflavin 0.00001

Flavine Adenine Dinucleotide•2Na 0.000106

Thiamine•HCl 0.00001

艾美捷CMRL-1066培養(yǎng)基文獻(xiàn)參考：

1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966).

2. Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O.

3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper and Row Publishers Inc., New York. Ed.: Parker, R.C.