化工儀器網(wǎng)
產(chǎn)品簡(jiǎn)介
ICG amine，吲哚菁綠-氨基，ICG-NH2 我公司是國(guó)內(nèi)生命科學(xué)和生物技術(shù)領(lǐng)域試劑供應(yīng)商，從成立初期就致力于為生命科學(xué)和生物技術(shù)領(lǐng)域提供優(yōu)質(zhì)產(chǎn)品和技術(shù)服務(wù)，力求將的產(chǎn)品和服務(wù)奉獻(xiàn)給我們的客戶(hù)。
詳細(xì)介紹
ICG amine，吲哚菁綠-氨基，ICG-NH2
英文名：ICG amine，ICG-NH2
中文名：吲哚菁綠-氨基
分子式：C47H56N4O4S
分子量：773.04
外觀：綠色粉末
溶解度：二氯甲烷
純度：95%+
結(jié)構(gòu)式：
ICG amine，吲哚菁綠-氨基，ICG-NH2
ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and emission maximum at 807 nm (10% v/vDMSO/PBS). Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm).
The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.
It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.
近紅外熒光染料Near IRDyes激發(fā)和發(fā)射波長(zhǎng)和顏色圖：
ICG衍生物產(chǎn)品：
ICG NHS ester 1622335-40-3
ICG-alkyne 1622335-41-4
ICG Maleimide 2143933-81-5
ICG-azide
ICG-DBCO
ICG Carboxylic acid
ICG Amine
ICG Thiol
ICG-CBT