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數(shù)量：
1
供應(yīng)商：
BPS Bioscience
細胞類型：
穩(wěn)轉(zhuǎn)細胞系
品系：
HepG2
組織來源：
/
相關(guān)疾病：
多種
物種來源：
human
免疫類型：
多種
器官來源：
無
運輸方式：
干冰
年限：
10
生長狀態(tài)：
良好
CAS號：
無
英文名：
CRISPRa (SAM) HepG2 Cell Line
注冊證號：
/
規(guī)格：
2管

This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.

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