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Description

The STAT3 Luciferase Reporter THP-1 cell line monitors the STAT3 (Signal Transducer and Activator of Transcription 3) signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, the endogenous transcription factor STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

These cells respond to IL-6 (Interleukin 6), to G-CSF (Granulocyte Colony-Stimulating Factor) and to IFN-γ (Interferon γ).

Figure 1: Illustration of STAT3 Luciferase Reporter THP-1 Cell Line reporter expression.
Created with BioRender.com

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Signal transducer and activator of transcription 3, Acute-phase response factor, APRF

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Product Data Gallery

Dose response to human IL-6 in STAT3 Luciferase Reporter THP-1 cells.

Cytokine response in STAT3 Luciferase Reporter THP-1 cells.

Dose-dependent response to human G-CSF in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of hIL-6-induced STAT3 Reporter Activity by JAK inhibitor CP 690,550 in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of hIL-6-induced STAT3 Reporter Activity by anti-IL-6 Receptor Antibody in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of G-CSF-induced STAT3 Reporter Activity by anti-G-CSF Receptor Antibody in STAT3 Luciferase Reporter THP-1 cells.

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