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            ARBOR ASSAYS 免疫測試產(chǎn)品 試劑盒-相關(guān)技術(shù)文章

            時(shí)間:2024/7/13閱讀:592
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            ARBOR ASSAYS 免疫測試產(chǎn)品 試劑盒


            Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測和免疫測試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測和酶活性試劑盒以定量生物分子。

            主要為臨床重要生物分子提供高質(zhì)量的檢測和免疫測試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測試劑盒、谷胱甘肽熒光檢測試劑盒、血紅蛋白比色檢測試劑盒等。種類多樣,操作簡單,靈敏度高。

            認(rèn)識到對靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測和免疫測定試劑盒。

            Arbor Assays公司的主要產(chǎn)品包括:

            DetectX® Kits

            Inhibitors & Activators

            AbX® Antibodies & Affinity Resins

            Detection Systems

            Components & Ancillary Reagents

            所需材料:

            類固醇標(biāo)準(zhǔn)品,用于檢測萃取效率

            ACS級乙醇(或乙酸乙酯)

            玻璃試管

            處理流程:

            確保樣品wan全干燥,并對樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測結(jié)果來確定提取效率,詳細(xì)信息請參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

            1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過被動干燥、溫和加熱(60°C)或冷凍的方式來進(jìn)行干燥處理。

            2.0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

            3.用力搖晃至少30分鐘。

            4.4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

            注:含有低水平分析物的樣品可以通過干燥提取液和在少量分析緩沖液中重懸,來達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存?;蛴靡掖?span>(100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

             5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μLAB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對于免疫分析,測定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測緩沖液進(jìn)行稀釋。

            6.根據(jù)試劑盒說明書對稀釋后的樣本進(jìn)行ELISA檢測。

             

            下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

            產(chǎn)品名稱

            貨號

            規(guī)格

            Goat Anti-Mouse IgG Fc, 25MG

            A008-25MG

            25MG

            Cyclic AMP Direct ELISA Kit (1 Plate)

            K019-H1

            1x96 well plate

            Corticosterone ELISA Kit (1 Plate)

            K014-H1

            1x96 well plate

            Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

            K027-H1

            1x96 well plate

            Aldosterone ELISA Kit (1 Plate)

            K052-H1

            1x96 well plate

            Urea Nitrogen (BUN) Detection Kit (2 Plate)

            K024-H1

            2x96 well plate

            Cortisol ELISA Kit (1 Strip plate)

            K003-H1

            1x96 well plate

            PGFM ELISA Kit (1 Plate)

            K022-H1

            1x96 well plate

            Triiodothyronine (T3) ELISA Kit (1 Plate)

            K056-H1

            1 Kit

            FRAP™ (Ferric Reducing Antioxidant Power) Detection   Kit (2 Plate)

            K043-H1

            2x96 well plate

            Hemoglobin Detection Reagent, 20ML

            C038-20ML

            20ML

            Ceruloplasmin Colorimetric Activity Kit (2 Plate)

            K035-H1

            2x96 well plate

            ThioStar™ Fluorescent Thiol Substrate, 100UG

            L002-100UG

            100UG

            Cyclic GMP Direct Chemiluminescent ELISA Kit (1   Plate)

            K020-C1

            1x96 well plate

            Progesterone ELISA Kit (1 Plate)

            K025-H1

            1x96 well plate

            Testosterone ELISA Kit (1 Plate)

            K032-H1

            1x96 well plate

            Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

            K036-H1

            1x96 well plate

            Thyroxine (T4) ELISA Kit (1 Plate)

            K050-H1

            1x96 well plate

            Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1   Plate)

            K051-H1

            1x96 well plate

            Tranylcypromine

            X042-1EA

            1EA

            Triiodothyronine (T3) ELISA Kit (5 Plate)

            K056-H5

            5x96 well plate

            Glutathione Colorimetric Detection Kit (4 Plate)

            K006-H1

            4x96 well plate

            Cyclic GMP Direct ELISA Kit (1 Plate)

            K020-H1

            1x96 well plate

            Progesterone ELISA Kit (5 Plate)

            K025-H5

            5x96 well plate

            Cyclic AMP Direct ELISA Kit (5 Plate)

            K019-H5

            5x96 well plate

            Estradiol ELISA Kit (1 Plate)

            K030-H1

            1x96 well plate

            Oxytocin ELISA Kit (1 Plate)

            K048-H1

            1x96 well plate

            Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

            K036-H5

            5x96 well plate

            Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5   Plate)

            K037-H5

            5x96 well plate

            Hemoglobin Standard, 300UL

            C037-300UL

            300UL

            DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

            C133-3ML

            3ML

            Creatinine Urinary Detection Kit (2 Plate)

            K002-H1

            2x96 well plate

            Butyrylcholinesterase Fluorescent Activity Kit (2   Plate)

            K016-F1

            2x96 well plate

            Corticosterone ELISA Kit, (5 Plate)

            K014-H5

            5x96 well plate

            Cyclic GMP Direct Chemiluminescent ELISA Kit (5   Plate)

            K020-C5

            5x96 well plate

            Superoxide Dismutase (SOD) Colorimetric Activity   Kit (2 Plate)

            K028-H1

            2x96 well plate

            Catalase Colorimetric Activity Kit (2 Plate)

            K033-H1

            2x96 well plate

            Glutathione Fluorescent Detection Kit (1 Plate)

            K006-F1

            1x96 well plate

            Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

            C128-625UL

            625UL

            Thyroxine (T4) ELISA Kit (5 Plate)

            K050-H5

            5x96 well plate

            Acetylcholinesterase Fluorescent Activity Kit (2   Plate)

            K015-F1

            2x96 well plate

            Creatinine Serum Kit (2 Plate)

            KB02-H1

            2x96 well plate

            Hydrogen Peroxide Fluorescent Detection Kit (2   Plate)

            K034-F1

            2x96 well plate

            Creatinine Serum Low Sample Volume Kit (384-well   Plate)

            KB02-H1D

            1x384 well plate

            Recombinant Obelin, 100UG

            L001-100UG

            100UG

            Dry DMSO, 20ML

            X022-20ML

            20ML

            Dry DMSO, 2ML

            X022-2ML

            2ML

            Urea Nitrogen Standard, 1ML

            C089-1ML

            1ML

            NADPH lyophilized, 1EA

            X045-1EA

            1EA

            DetectX® PGE2 Conjugate, 13ML

            C179-13ML

            13ML

            17-Hydroxyprogesterone   ELISA Kits

            K053-H

            1 Kit

            Arg8-Vasopressin (AVP) ELISA Kits

            K049-H

            1 Kit

            Arg8-Vasopressin (AVP) Chemiluminescent   ELISA Kits

            K049-C

            1 Kit

            Androstenedione ELISA Kits

            K070-H

            1 Kit

            Aldosterone Chemiluminescent ELISA Kits

            K052-C

            1 Kit

            Allopregnanolone ELISA Kits

            K061-H

            1 Kit

            Adrenocorticotropic Hormone (ACTH) ELISA   Kits

            K072-H

            1 Kit

            Alkaline Phosphatase (ALP) Colorimetric   Activity Kit

            K082-H1

            1 Kit

            3,3-Cyclic GAMP ELISA Kits

            K073-H

            1 Kit

            20-Hydroxyecdysone ELISA Kit

            K066-H

            1 Kit

            2,3-Cyclic GAMP STING-Based FRET Detection Kits

            K081-F

            1 Kit

            2,3-Cyclic GAMP ELISA Kits

            K067-H

            1 Kit

            Cyclic GMP Direct ELISA Kits – Improved   Sensitivity

            K065-H

            1 Kit

            Endothelin-1 (ET-1) ELISA Kit

            K045-H1

            1 Kit

            Estrone-3-Sulfate (E1S) ELISA Kits

            K038-H

            1 Kit

            Assay Buffer, 5X

            X065-110ml

            110ml

            Blocking Buffer, 10X

            X109-25ml

            25ml

            Goat anti-Rabbit IgG Coated Plate

            X016-1EA


            HRP Conjugate Diluent

            X076-300ml

            300ml

            TMB Substrate

            X019-55ml

            55ml

            Mesotocin Solution (500ng/mL)

            X127-625UL


            5-Azacytidine

            P012-50MG

            50mg

             

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