人尿微量白蛋白(mALB）ELISA試劑盒
Human microalbunminuria(mALB)ELISA Kit
試劑盒原理：用于測定人尿液相關(guān)液體樣本尿微量白蛋白(mALB）含量。本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中人尿微量蛋白(mALB)水平。用純化的人尿微量蛋白(mALB)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入尿微量蛋白
(mALB)，再與HRP 標(biāo)記的mALB 抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB 顯色。TMB 在HRP 酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的尿微量蛋白(mALB)呈正相關(guān)。用酶標(biāo)儀在450nm 波長下測定吸光度（OD 值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中人尿微量蛋白(mALB)濃度。
Principle of the assay:
This kit allows for the determination of mALB concentrations in Human
Urine and other biological fluids.The kit assay Human mALB level in the sample，use Purified Human mALB antibody to coat microtiter plate wells, make solid-phase antibody, then add mALB to wells, Combined mALB antibody which With HRP labeled , become
antibody - antigen - enzyme-antibody complex, after washing Compley, Add
TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of mALB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司