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不同電擊管的電壓參數(shù)需要換算，時間相同。以20ul電擊管參數(shù)為基準，100ul比20ul約上調(diào)10V；200ul是20ul的約2.4倍；1000ul是20ul約2.5倍；120ul的是20ul的約1.4倍，600ul的是20ul的約1.6倍。下面是轉(zhuǎn)換示例：
20ul：500V
100ul: 510V （20ul電壓+10V）
200ul：1200V （20ul電壓的2.4倍）
1000ul：1250V （20ul電壓的2.5倍）
120ul：700V （20ul電壓的1.4倍）
600ul：800V （20ul電壓的1.6倍）
*部分單核細胞相關的細胞系，電轉(zhuǎn)質(zhì)粒后幾乎所有電轉(zhuǎn)成功的細胞會死亡，而電轉(zhuǎn)mRNA，siRNA和Cas9 RNP沒關系。Celetrix公司專門開發(fā)了試劑盒可以幫助單核細胞類的細胞系進行質(zhì)粒電轉(zhuǎn)，目前驗證過THP-1和U937這兩種細胞系電轉(zhuǎn)效果非常好，而Molm-13和Sup-M2細胞電轉(zhuǎn)質(zhì)粒細胞死亡問題還沒有解決。
| Cell Type | Mode | 20ul, 100ul | 200ul, 1ml |
|---|---|---|---|
| Human PBMC | PBMC | 1200 -1400 | NA |
| Human T cells, unstimulated | PBMC | 1300 -1450 | NA |
| Human B cells, unstimulated | PBMC | 1300 -1450 | NA |
| Human NK cells, unstimulated | PBMC | 1300 -1450 | NA |
| Human Neutrophil | PBMC | 1300 -1450 | NA |
| Mouse lymph node primary T cell | PBMC | 1300 -1450 | NA |
| Mouse primary spleen cells | PBMC | 1300 -1450 | NA |
| Mouse bone marrow monocyte | PBMC | 1300 -1450 | NA |
| Human Cultured T cells | Cell Line | 480-540 | 1150-1300 |
| Human Cultured NK cells | Cell Line | 480-540 | 1200-1300 |
| Human iPSC | Cell Line | 500-540 | 1200-1300 |
| Human MM1.S | Cell Line | 520-560 | 1250-1350 |
| Human adipose MSC | Cell Line | 500-540 | 1200-1300 |
| Human bone marrow MSC | Cell Line | 500-540 | 1200-1300 |
| Human cord blood MSC | Cell Line | 500-540 | 1200-1300 |
| Human ESC derived neural stem cell | Cell Line | 520-560 | 1250-1350 |
| Human primary hepatocyte | Cell Line | 480-520 | 1150-1250 |
| Human primary Dendritic cells | Cell Line | 480-520 | 1150-1200 |
| Human neural progenitor ReNCell VM | Cell Line | 520-560 | 1250-1350 |
| Mouse iPSC | Cell Line | 480-520 | 1150-1250 |
| Mouse ESC | Cell Line | 480-520 | 1150-1250 |
| Mouse lymph node Cultured T cell | Cell Line | 500-540 | 1200-1300 |
| Mouse peritoneal Macrophage | Cell Line | 540-580 | 1350-1400 |
| Primary mouse cortical neurons | Cell Line | 520-560 | 1350-1400 |
| Rat primary cortex neural progenitor cell | Cell Line | 520-560 | 1250-1350 |
| 293 | Cell Line | 440-480 | 1050-1150 |
| 293T | Cell Line | 460-500 | 1050-1150 |
| A20 | Cell Line | 540-560 | 1250-1350 |
| A549 | Cell Line | 540-560 | 1250-1350 |
| ADSCs | Cell Line | 500-520 | 1200-1300 |
| ARPE-19 | Cell Line | 520-540 | 1250-1350 |
| BaF3 | Cell Line | 520-540 | 1250-1350 |
| BJ | Cell Line | 500-520 | 1250-1400 |
| CAL51 | Cell Line | 500-520 | 1250-1350 |
| CHO-K1 | Cell Line | 560-600 | 1250-1400 |
| CHO-S | Cell Line | 520-580 | 1250-1400 |
| DEL | Cell Line | 500-540 | 1200-1300 |
| DLD1 | Cell Line | 500-520 | 1200-1300 |
| EMT6 | Cell Line | 500-520 | 1200-1300 |
| HCT116 | Cell Line | 540-560 | 1200-1300 |
| Hela | Cell Line | 480-520 | 1250-1350 |
| Hepa1-6 | Cell Line | 500-520 | 1200-1300 |
| Hep3b | Cell Line | 500-540 | 1100-1200 |
| HSF | Cell Line | 520-540 | 1250-1350 |
| HUC-Maaa13-P3 | Cell Line | 520-540 | 1250-1350 |
| HUVEC | Cell Line | 520-560 | 1250-1350 |
| H1975 | Cell Line | 520-560 | 1250-1350 |
| H22 | Cell Line | 500-520 | 1200-1300 |
| H226 | Cell Line | 520-540 | 1250-1350 |
| H9C2 | Cell Line | 500-540 | 1350-1400 |
| HL-60 * | Cell Line | 520-560 | 1250-1350 |
| iMC | Cell Line | 500-540 | 1200-1300 |
| Jurkat | Cell Line | 500-540 | 1200-1350 |
| Karpas299 | Cell Line | 520-560 | 1250-1350 |
| K562 | Cell Line | 480-520 | 1200-1300 |
| MC38 | Cell Line | 500-520 | 1200-1300 |
| MCF-7 | Cell Line | 500-520 | 1200-1300 |
| MDA-MB-231 | Cell Line | 500-520 | 1220-1300 |
| Molm-13 * | Cell Line | 500-540 | 1250-1350 |
| Nalm-6 | Cell Line | 520-540 | 1250-1350 |
| NK-92 | Cell Line | 440-460 | 1150-1200 |
| Raji | Cell Line | 520-540 | 1250-1350 |
| RAW264.7 | Cell Line | 520-540 | 1250-1350 |
| SF9 insect cell | Cell Line | 480-520 | 1200-1300 |
| SK-HEP-1 | Cell Line | 480-520 | 1220-1300 |
| SP2/0 | Cell Line | 480-500 | 1150-1200 |
| SUP-M2 * | Cell Line | 520-560 | 1250-1350 |
| Swiss-3T3 | Cell Line | 520-540 | 1250-1350 |
| p3X63Ag8.653 | Cell Line | 500-540 | 1220-1300 |
| THP-1 * | Cell Line | 500-540 | 1250-1300 |
| U251 | Cell Line | 480-520 | 1250-1400 |
| U937 * | Cell Line | 500-540 | 1250-1300 |
*Many monocyte or macrophage related cell lines such as THP-1, U937, HL-60 and Molm13 will die out within two to three days after plasmid electroporation. These cells have no problem with mRNA, siRNA and Cas9 RNP electroporation. The efficiency would be nearly 100% and cell survival is not affected. Celetrix has developed the THP-1 Helper Kit for plasmid electroporation of monocytic cell lines. A significant portion of THP-1 and U937 cells can survive and grow with the helper kit, enabling easy generation of stable cell lines. Our THP-1 Helper Kit does not help cell lines such as Molm13 and SUP-M2 with plasmid electroporation and we are actively researching the solutions.
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