The basement membrane matrix is a soluble basement membrane preparation extracted from the tumors of EHS mice rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG) and nestin, as well as growth factors such as TGF beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors.

The Ceturegel™ basement membrane matrix developed and produced by YEASEN Biological does not contain LDEV (Lactate Dehydrogenase Enhancing Virus) and has ultra-low endotoxin content. And after mycoplasma detection to ensure no mycoplasma contamination, including different types such as basic concentration, high concentration, and low growth factor.

Applications

Product features

High safety: no LDEV (lactate dehydrogenase increased virus)

Concentration diversity: the concentration range is between 8~20 mg/ml

Good batch stability: strict production quality inspection process to ensure stable performance between batches

Low endotoxin: endotoxin content <8 EU/ml

Contamination detection: no mycoplasma, bacteria, and fungi residues have been detected

High single batch output: single batch output is above 50L level

Compatibility: Compatible with any type of cell culture medium

Application validation

Fig. Results of crystal violet staining after cell invasion

Fig. HUVEC angiogenic bright field and fluorescence results

Fig. H9 stem cell culture

Fig： Hepg2 cell 3D culture results after 4 days

Fig: HepG2 subcutaneous tumor model establishment results

Fig: The results of in vitro culture of matrix glue mixed with mouse small intestinal crypts

Fig: Ovarian cancer organoid image cultured for 6 days  Fig:   cervical cancer organoids for 4 days

FAQ

1. What is the reason for the color difference (light yellow to dark red) of the obtained substrate？

For the Ceturegel™ basement membrane matrix containing phenol red, it is mainly caused by the interaction of phenol red and bicarbonate with CO2, but the color difference will be reduced after equilibration with 5% CO2. After freezing and thawing, shake the vial gently to disperse the Ceturegel™ basement membrane matrix evenly.

2. What matters should be paid attention to in the operation of Ceturegel™ basement membrane matrix？

All operations should be performed in a sterile environment, and a pre-cooled pipette should be used to ensure that the Ceturegel™ basement membrane matrix is homogenized.

3. How to freeze and store Ceturegel™ basement membrane matrix for use？

The frozen and thawed Ceturegel™ Matrix LDEV-Free Ceturegel™ basement membrane matrix can be distributed in multiple small tubes. All distributions should be in pre-cooled cryovials, which should be quickly frozen and stored to avoid multiple freezing and thawing. All items involved should be pre-cooled before use. Use pre-cooled pipettes, tips, and small tubes to handle Ceturegel™ basement membrane matrix.

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