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            當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>昆蟲卵巢細(xì)胞>>CRL-1711 SF9 昆蟲卵巢細(xì)胞

            CRL-1711 SF9 昆蟲卵巢細(xì)胞

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            CRL-1711 SF9 昆蟲卵巢細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

            CRL-1711 SF9 昆蟲卵巢細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


            CRL-1711 SF9 昆蟲卵巢細(xì)胞 的詳細(xì)介紹


            ATCC® Number: CRL-1711™    Price: $275.00
            Designations: Sf9

            Depositors: G Smith, C Cherry, MD Summers

            Biosafety Level:1

            Shipped: frozen

            Medium & Serum: See Propagation

            Growth Properties:mixed: adherent/suspension

            Organism:Spodoptera frugiperda (fall armyworm)

            Morphology:epithelial
            CRL-1711 SF9 昆蟲卵巢細(xì)胞


            Source:Organ: ovary

            Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


            Isolation: Isolation date: 1983

            Applications:transfection host (Roche FuGENE® Transfection Reagents

            technology from amaxa)



            Virus Susceptibility:Nuclear polyhedrosis virus, Autographa californica

            St. Louis encephalitis virus



            Age: pupa

            Gender: female

            Comments:This line can be used to replicate baculovirus expression vectors.

            It is important to use the medium described below.



            Propagation: ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with:

            500 mg/L calcium chloride

            2800 mg/L potassium chloride

            3330 mg/L lactalbumin hydrolysate

            3330 mg/L yeastolate

            10% Heat-inactivated fetal bovine serum (previously tested for insect cell culture)


            Atmosphere: air, 100%
            Temperature: 28.0°C
            Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.


            Subculturing: Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).
            Subc*tion Ratio: A subc*tion ratio of 1:5 or greater is recommended
            Medium Renewal: Every 2 to 3 days


            Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
            Storage temperature: liquid nitrogen vapor phase


            References:22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913

            23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453

            23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519

            32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separay cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050

            32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063

            32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863

            32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109

            32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508

            33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802

            33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793

            33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736

            33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509




















            CRL-1711 SF9 昆蟲

            CRL-1711 SF9 昆蟲卵巢細(xì)胞,ATCC

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