Catalogue Number : 05121-77

CD3產(chǎn)品描述：OKT3單克隆抗體的特異性反應(yīng)的CD3 / T淋巴細(xì)胞抗原受體復(fù)合物的ε鏈。CD3復(fù)合物含有γ，δ，和ε鏈，和它的TCR復(fù)雜的部分，由所有成熟T淋巴細(xì)胞和胸腺細(xì)胞系的表達。CD3增強的信號轉(zhuǎn)導(dǎo)的抗原識別。

PeproTech公司的BioGems產(chǎn)品OKT3抗體是一種免疫抑制，這已被證明是一種有效的治療劑，肝，心，腎移植排斥反應(yīng)。

Additional Information

Clone
OKT3
Format:
PE-Cy7
Applications
FC
Reactivity
Human

Isotype:
Mouse IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: Treg cells, T Cells, NK and NKT Cells, Th17 cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Adair, J. R., Athwal, D. S., Bodmer, M. W., Bright, S. M., Collins, A. M., Pulito, V. L., ... & Jolliffe, L. K. (1994). Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Human Antibodies, 5(1), 41-47.

Kap, Y. S., van Meurs, M., van Driel, N., Koopman, G., Melief, M. J., Brok, H. P., ... & A't Hart, B. (2009). A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. Journal of Histochemistry & Cytochemistry, 57(12), 1159-1167.

Anti-Human CD3 PE-Cy7
Technical Data Sheet
Catalogue Number : 05121-77
RUO: For Research Use Only. Not for use in diagnostic procedures.
Product Information
Clone: OKT3
Format/Conjugate: PE-Cy7
Concentration: 5 uL (0.5 ug)/test
Reactivity: Human
Laser: Blue (488nm)
Peak Emission: 785nm
Peak Excitation: 496nm
Filter: 780/60
Brightness (1=dim,5=brightest): 4
Isotype: Mouse IgG2a, kappa
Formulation: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Storage: Product should be kept at 2-8°C and protected from prolonged exposure to light.
Applications: FC
Description
The OKT3 monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. The
CD3 complex contains γ, δ, and ε chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and
by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.
The OKT3 antibody is an immunosuppressive, which has proven to be an effective therapeutic agent in liver, heart, and
renal allograft rejection.
Preparation & Storage
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Application Notes
The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. The antibody
can be used at less than or equal to 5 μL per test. A test is the amount of antibody required to stain a cell sample in
the final volume of 100 μL.

重慶市華雅干細(xì)胞技術(shù)有限公司代理的PeproTech公司旗下的BioGems產(chǎn)品，咨詢：

           :
References
1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York
2.
Knapp W;(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.
3.
Adair, J. R., Athwal, D. S., Bodmer, M. W., Bright, S. M., Collins, A. M., Pulito, V. L., ... ; Jolliffe, L. K. (1994). Humanization of the murine
anti-human CD3 monoclonal antibody OKT3.;Human Antibodies,;5(1), 41-47.
4.
Kap, Y. S., van Meurs, M., van Driel, N., Koopman, G., Melief, M. J., Brok, H. P., ... ; A't Hart, B. (2009). A monoclonal antibody selection for
immunohistochemical examination of lymphoid tissues from non-human primates.;Journal of Histochemistry ; Cytochemistry,;57(12), 1159-1167.

PeproTech公司旗下的BioGems產(chǎn)品的其他相關(guān)信息如下：

Additional OKT3 Formats 

Cat.No. Format Starting From
貨號：05121-20 Purified

貨號：05121-25 SAFIRE Purified

貨號：05121-40 BG Violet 450

貨號：05121-60 PE

貨號：05121-80 APC

貨號：05121-50 FITC

貨號：05121-70 PerCP-Cy5.5

貨號：05121-77 PE-Cy7