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            上海宸喬生物科技有限公司>>Ultrasep ES液相色譜柱>>Clinical Chemistry(臨床化學(xué))>>Amino Acids and Peptides

            Amino Acids and Peptides

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            • Amino Acids and Peptides

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            The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

            詳細(xì)介紹

            Amino Acid and Peptide Analysis

             

            UltraSep ESD AP

              

            The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

            Comparison of different procedures for derivatization

            Procedure

            Detection

            Limit of detection

            Remarks

            DABS

            Absorption
            436 nm

            < 1 pmol

            Stable derivatives; primary and secondary amino acids can be determined

            OPA

            Fluorescence
            330/440 nm

            < 1 pmol

            Pro, Hypro and Cys cannot be determined 

            FMOC

            Fluorescence
            265/315 nm

            < 1 pmol

            His and Cys will be detected only in part

            DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
            OPA = o-Phthalaldehyde
            FMOC = 9-Fluorenylmethoxycarbonylchloride

             

            Examples of Analyzed Compounds

            Analyzed in Application

            Go to

             

            Alanine (Ala)

            AA

             

             

            Arginine (Arg)

            AA

            AA

             

            Aspartic acid (Asp)

            AA

             

             

            Cystine (Cys2)

            AA

            EPHARM6

             

            Glutamic acid (Glu)

            AA

             

             

            Glycine (Gly)

            AA

             

             

            Glutathione

            EPHARM6

             
             

            Histidine (His)

            AA

             

             

            Isoleucine (Ile)

            AA

             

             

            Leucine (Leu)

            AA

             

             

            Lysine (Lys)

            AA

             

             

            Methionine (Met)

            AA

             

             

            Ornithine (Orn)

            AA

             

             

            Phenylalanine (Phe)

            AA

             

             

            Proline (Pro)

            AA

             

             

            Serine (Ser)

            AA

             

             

            Threonine (Thr)

            AA

             

             

            Tyrosine (Tyr)

            AA

             

             

            Valine (Val)

            AA

             

             

            COLUMNS and  PRICES  ()
             Column size (mm)
            250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
            UltraSep ESD AP360.00340.00355.00320.00295.00290.00
             

            Size (mm)

             
            10 x 410 x 310 x 2
            Precolumn cartridges
            (Price per pack = 5 pieces)
            170.00170.00170.00

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