狠狠色丁香久久综合婷婷亚洲成人福利在线-欧美日韩在线观看免费-国产99久久久久久免费看-国产欧美在线一区二区三区-欧美精品一区二区三区免费观看-国内精品99亚洲免费高清

CUTANA pAG-Tn5 for CUT Tag 試劑返回列表頁

舉報評價

參考價:9540

具體成交價以合同協(xié)議為準

型號
品牌
EpiCypher
廠商性質
代理商
所在地
深圳市

規(guī)格

50 Reactions 9540元 59件可售

在線詢價加入對比查看聯(lián)系電話

更新時間：2024-05-22 15:04:58瀏覽次數(shù)：495次

聯(lián)系我時，請告知來自化工儀器網(wǎng)

產(chǎn)品分類品牌分類

表觀遺傳學

全部產(chǎn)品列表

產(chǎn)品簡介

CAS	請詢	純度	請詢
分子量	請詢	分子式	請詢
供貨周期	兩周	規(guī)格	50 Reactions
貨號	15-1017	應用領域	生物產(chǎn)業(yè),綜合
主要用途	科研

詳情介紹

CUTANA pAG-Tn5是靶向剪切及轉座酶(CUT&Tag)技術中進行高效繪制染色質特征的關鍵試劑。與ChIP-seq相比，CUT&Tag在降低細胞需求量和測序深度的信噪比方面進行了顯著改進。CUTANA pAG-Tn5是一種高活性的E. coli轉座酶突變體(Tn5)與蛋白A/G的融合產(chǎn)物，可與來自多種物種宿主的靶抗體兼容。它也沒有表位標簽，使其適合于標簽介導的CUT&Tag(如FLAG、HA、TY1、V5等)。該產(chǎn)品經(jīng)過高度純化，已去除污染的E. coli DNA，并在細胞數(shù)量少的情況下進行復雜分析。為了每次實驗的標準化以及抗體驗證和反應監(jiān)測，推薦使用SNAP-CUTANA™ nucleosome spike-ins(如EpiCypher 19-1002)。Tn5帶有mosaic 接頭，可在CUT&Tag中使用。

Adapters

Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’

Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’

Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’

組分

50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

保存條件

Stable for one year at -20℃ from date of receipt. The protein is not subject to freeze/thaw under these conditions.

數(shù)據(jù)示例

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 1:

Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Uncharged pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA, Tn5 dimerizes to a final complex weight of 191 kDa.

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 2:

Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 3:

CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control.

訂購詳情