CD163 M130抗原(鼠單克隆抗體)

廣州健侖生物科技有限公司

CD163是一種I型膜蛋白，又稱為M130抗原、Ber-Mac3、Ki-M8或SM4，屬于富含半胱氨酸的清道夫受體家族。表達于單核/巨噬細胞系統(tǒng)，但不表達于淋巴濾泡的套區(qū)和生發(fā)中心。主要用于研究單核細胞和巨噬細胞。

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【產品介紹】

細胞定位：細胞膜/細胞漿

克隆號：MRQ-26

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：骨髓單核細胞性白血病，炎癥組織，淋巴結（單核細胞，巨噬細胞）

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD163 M130抗原(鼠單克隆抗體)

2、操作步驟
（1）用PBS稀釋SPA成5Ps/ml，包被聚苯乙烯反應板微孔，每孔0.1ml（對照孔不包被），置4℃過夜后洗滌3次備用；
（2）待測血清0.05ml加PBS0.15ml和5%PEG0.2ml混勻，4℃過夜后1600r/min離心20min，棄上清，沉淀用2.5%PEG洗2次，加入PBS0.2ml和BSA緩沖液0.2ml，混勻，置37℃水浴30min，搖動，使*溶解；
（3）將已溶解的待測血清沉淀物加至上述包被孔和對照孔中，置37℃60min，洗3次；各孔加底物溶液（OPD—H202）0.1ml，37℃ 20min使呈色。每孔加2mol/LH2S041滴終止反應。置酶標儀492nm測各孔吸光值；
（4）標準曲線制備：取正常人血清0.2ml，加熱聚合人IsC（120ug/ml）0.2ml，再加PBS0.4ml和5%PEG0.8ml，置4℃過夜。同時做不加熱聚合人耽的正常血清對照，以排除血清中干擾因素。沉淀清洗同標本操作。用稀釋的BSA緩沖液（加等量0.01mol/LpH7.4PBS）1.6ml溶解并稀釋成120、60、30、15、7.5ug/ml，與待測血清同法操作，制成工作標準曲線；
（5）結果判斷：從待測血清吸光度值查標準曲線，即可換算成相當于熱聚合人IgG的CIC含量（ug/ml）。以高于正常對照X+2S，即大于28.4ug/ml為陽性。
3、注意事項
（1）熱聚合人IgC應分裝貯存于-20℃，不宜反復凍融，否則易解聚；
（2）PEG的濃度影響CIC沉淀量，須嚴格配制。
實驗材料
1、活材料：培養(yǎng)3d的黑曲霉（Aspergillus niger）、根瘤菌（Rhizobium sp.）、培養(yǎng)24h的大腸桿菌（E. coli）、巴斯德梭菌（Clostridium pasteurianum）、枯草桿菌（Bacillus subtilis）、培養(yǎng)5d的吸水鏈霉菌“5102”（Sterptomyces hygroscopicus “5102”）、培養(yǎng)24～48h的金黃色葡萄球菌（Staphylococcus aureus）及黏質沙雷氏菌（Serratia marcescens）的斜面菌種；其它自選的微生物材料，包括實驗室已有的和自己從環(huán)境中分離得到的均可。

CD163 M130抗原(鼠單克隆抗體)

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

2, steps
(1) SPA was diluted with PBS to 5Ps / ml, coated polystyrene microplate wells, each hole 0.1ml (control hole not coated), set at 4 ℃ overnight after washing 3 times spare;
(2) Test serum 0.05ml plus PBS0.15ml and 5% PEG0.2ml mix, overnight at 4 ℃ 1600r / min after centrifugation 20min, the supernatant was discarded, the precipitate was washed twice with 2.5% PEG, PBS0.2ml and BSA Buffer 0.2ml, mix, set 37 ℃ water bath 30min, shake, so compley dissolved;
(3) The dissolved serum precipitate to be added to the above coated wells and control wells, set 37 ℃ 60min, washed three times; each well plus substrate solution (OPD-H202) 0.1ml, 37 ℃ 20min Coloring. Each hole plus 2mol / LH2S041 drops to terminate the reaction. Set microplate reader 492nm absorbance of each well;
(4) Preparation of standard curve: Take normal human serum 0.2ml and heat the polymerized human IsC (120ug / ml) 0.2ml, add PBS0.4ml and 5% PEG0.8ml and set overnight at 4 ℃. At the same time do not heat the aggregation of people delayed normal serum control to exclude interference in serum. Precipitation wash with the specimen operation. With diluted BSA buffer (plus the same amount of 0.01mol / LpH7.4PBS) 1.6ml dissolved and diluted to 120,60,30,15,7.5 ug / ml, with the test serum in the same manner, the working standard curve;
(5) Judgment of the result: The standard curve of absorbance of serum to be tested can be converted into CIC content (ug / ml) equivalent to thermal polymerization human IgG. It is higher than the normal control X + 2S, ie greater than 28.4 ug / ml.
3, Matters needing attention
(1) Thermal polymerization of human IgC should be stored in aliquots stored at -20 ℃, should not be repeated freezing and thawing, or easy depolymerization;
(2) The concentration of PEG affect the amount of CIC precipitation, to be strictly formulated.
Experimental Materials
1, Live materials: Aspergillus niger, Rhizobium sp. Cultured for 3 days, E. coli cultured for 24h, Clostridium pasteurianum, Bacillus subtilis, , Sterdomyces hygroscopicus 5102 "cultured for 5 days, Staphylococcus aureus and Serratia marcescens c*ted for 24-48 hours. Other optional Microbial materials, including those already in the laboratory and isolated from the environment.