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17504-044B-27® 添加劑是無血清添加劑
杭州仟諾生物科技有限公司17504-044B-27® 添加劑是無血清添加劑 N2等常用試劑大量現(xiàn)貨備貨，到貨
B-27® 添加劑是無血清添加劑，用于海馬神經(jīng)元和其他中樞神經(jīng)系統(tǒng)（CNS）神經(jīng)元的生長和保持其短期或長期活性。B-27® 添加劑是50X 的液體，可以與Neurobasal® 培養(yǎng)基或Neurobasal®-A 培養(yǎng)基組合使用，用于神經(jīng)細(xì)胞培養(yǎng)而不需要再添加飼養(yǎng)層細(xì)胞。
B-27® 添加劑適和應(yīng)用于大多數(shù)神經(jīng)細(xì)胞培養(yǎng)。對于特別的應(yīng)用，我們提供 其他配方的B-27® 添加劑.
質(zhì)量控制檢測
B-27® 添加劑 進行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海馬神經(jīng)元生長檢測。 通過了細(xì)菌和真菌的檢測. B-27® 也做了1X 濃度的內(nèi)毒素水平測試。
僅供研究使用。除非另有說明，不得用于任何動物或人類治療或診斷。
詳細(xì)說明
B-27® 添加劑是無血清添加劑
常用規(guī)格
| Cell Type: | Neuronal Cells |
| Product Size: | 10 ml |
| Classification: | Serum-Free |
| Other Additives: | Vitamin A, Antioxidant Cocktail, Insulin |
| Purity or Quality Grade: | Cell Culture Grade |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Dry Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
引文和參考文獻 （17）
D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.
作者
Congar P; Bergevin A; Trudeau LE
雜志
J Neurophysiol （2002） 87:1046-1102
產(chǎn)品用途
Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）.
ID:
PN64425
Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.
作者
Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;
雜志
Neuron （2002） 33:789-804
產(chǎn)品用途
Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.
ID:
PN47418
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.
作者
Kabos Peter; Kabosova Andrea; Neuman Toomas;
雜志
J Biol Chem （2002） 277:8763-8769
產(chǎn)品用途
Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）
ID:
PN63471
Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
作者
Lesuisse Christian; Martin Lee J;
雜志
J Neurobiol （2002） 51:9-23
產(chǎn)品用途
A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.
ID:
PN60461
An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.
作者
Joseph-Bravo Patricia; Pérez-MartÃnez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;
雜志
Brain Res Brain Res Protoc （2002） 9:93-104
產(chǎn)品用途
Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties
ID:
PN62028