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測(cè)量應(yīng)用案例-32- 90Plus
閱讀：275 發(fā)布時(shí)間：2015-6-2
文獻(xiàn)名： Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tip—effects of oxidative stress generation and biouptake
作者： A. Rajeshwari1 & S. Kavitha1 & Sruthi Ann Alex1 & Deepak Kumar1 & Anita Mukherjee2 & Natarajan Chandrasekaran1 & Amitava Mukherjee1
1 Centre for Nanobiotechnology, VIT University, Vellore, India
2 Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, India
摘要：The commercial usage of Al2O3 nanoparticles (Al2O3 NPs) has gone up significantly in the recent times, enhancing the risk of environmental contamination with these agents and their consequent adverse effects on living systems. The current study has been designed to evaluate the cytogenetic potential of Al2O3 NPs in Allium cepa (root tip cells) at a range of exposure concentrations (0.01, 0.1, 1, 10, and 100 μg/mL), their uptake/internalization profile, and the oxidative stress generated. We noted a dose-dependent decrease in the mitotic index (42 to 28 %) and an increase in the number of chromosomal aberrations. Various chromosomal aberrations, e.g. sticky, multipolar and laggard chromosomes, chromosomal breaks, and the formation of binucleate cells, were observed by optical, fluorescence, and confocal laser scanning microscopy. FT-IR analysis demonstrated the surface chemical interaction between the nanoparticles and root tip cells. The biouptake of Al2O3 in particulate form led to reactive oxygen species generation, which in turn probably contributed to the induction of chromosomal aberrations.
關(guān)鍵詞：Al2O3 NPs . Allium cepa . Aberration . Biouptake . SOD