B16-GFP小鼠黑色素瘤細(xì)胞-綠色標(biāo)記 處理步驟被

細(xì)胞在培養(yǎng)過程中如果污染了，不要試圖去除污染（非常重要的細(xì)胞株才考慮去除污染），一般的原則是應(yīng)將污染的細(xì)胞棄去。
1)細(xì)菌污染的排除：可使用大劑量抗生素沖擊法，向污染的細(xì)胞培養(yǎng)液中加入大劑量的抗革蘭氏陽性和陰性細(xì)菌的抗生素，一般采用5～10倍于常用量進(jìn)行用藥，加入高濃度抗生素后作用24-48小時，再換入常規(guī)的培養(yǎng)液，有時可以奏效。
2)支原體的排除：卡那霉素、慶大霉素、支原體去除因子(MRA)、卷須霉素及BM-Cycline等對抗支原體是有活性的，可使用它們抑制或消除支原體。
推薦培養(yǎng)基：（AM, Cat. No. 1801）
儲存：液氮
運(yùn)輸：干冰
用途：科研
保存條件：4℃保存一年；-20℃長期保存。

B16細(xì)胞的生長狀態(tài)：

公司所提供的實(shí)驗(yàn)B16BL6小鼠黑色素瘤細(xì)胞，不能用于人體實(shí)驗(yàn)和臨床診斷、治療。我司細(xì)胞研究中心承諾盡zui大努力對實(shí)驗(yàn)細(xì)胞資源相關(guān)信息進(jìn)行及時更新。但限于當(dāng)時的技術(shù)條件，中心不保證其準(zhǔn)確性，從文獻(xiàn)等處引用的信息本中心未進(jìn)行核實(shí)，僅供參考。
通過GFAP抗體熒光免疫方法驗(yàn)證，每管含有細(xì)胞數(shù)>1x106 cells/ml，經(jīng)測試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。密度超過90%，品牌認(rèn)證，我們現(xiàn)貨供應(yīng)細(xì)胞株、細(xì)胞系多達(dá)4000多種產(chǎn)品，您可放心采購。

A2780/Taxol-Luc，Luciferase螢火蟲酶熒光穩(wěn)定表達(dá)細(xì)胞株人卵巢癌紫杉醇耐藥株-NTCC保藏中心
【Organism物種來源】Animal 
【Tissue組織來源】 
【Cell Type細(xì)胞類型】 
【Product Format產(chǎn)品狀態(tài)】 frozen/live culture
【Morphology細(xì)胞形態(tài)】 
【Culture Properties細(xì)胞特性】 
【Biosafety Level生物安全級別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細(xì)胞源疾病】
【Age年齡】 
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】 
【Images細(xì)胞圖片】 Cell Micrograph
【Derivation衍生細(xì)胞】
【Clinical Data臨床數(shù)據(jù)】 
【Comments其他描述】 
【Complete Growth Medium*培養(yǎng)基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
【Subculturing傳代方法】 
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】 
【Freeze Medium凍存培養(yǎng)基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養(yǎng)條件】 
【Atmosphere培養(yǎng)環(huán)境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養(yǎng)溫度】 37°C
【STR Profile圖譜】 
【Population Doubling Time倍增殖時間】
【References參考文獻(xiàn)】